Preparation and some properties of highly purified human serum kallikrein

Clinica Chimica Acta; International Journal of Clinical Chemistry
G A YarovayaM Kawalec

Abstract

A 3000--6000-fold purified kallikrein was obtained from human serum in 10--25% yield by chromatography on QAE-Sephadex A-50, Molselect CM-50 and on soybean trypsin inhibitor (SBTI)-AH-Sepharose 4-B. The enzyme had a specific activity of 14--23 U, as measured by BAEE hydrolysis. Some properties of highly purified kallikrein are described.

References

Jun 25, 1999·Biopolymers·Y OkadaS Okamoto
Aug 18, 1982·Clinica Chimica Acta; International Journal of Clinical Chemistry·G A YarovayaV N Orekhovich
Aug 31, 1984·Clinica Chimica Acta; International Journal of Clinical Chemistry·J KawiakG A Yarovaya

Citations

Nov 1, 1974·Archives of Biochemistry and Biophysics·C SampaioE Shaw
Jan 1, 1972·The Journal of Experimental Medicine·K D Wuepper, C G Cochrane
May 1, 1970·British Journal of Pharmacology·D J McConnell, B Mason

Related Concepts

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Glycine max
Trypsin Inhibitor, Kunitz Soybean
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Sephadex

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