PMID: 9663847Jul 15, 1998Paper

Preparation and transplantation of photoreceptor sheets

Current Eye Research
J C HuangM A Zarbin

Abstract

Photoreceptor (PR) transplantation may be a treatment for blindness secondary to PR degeneration. We studied different technical aspects of PR-sheet preparation. Geographic variation in the thickness of the cat PR layer (from the outer segments to the outer plexiform layer) and inner retina (width of the remainder of the retina) was studied. PR sheets (cat and human) were prepared through gelatin embedding and subsequent vibratoming or excimer laser ablation. Cat PR sheets were evaluated after transplantation. The thickness of the cat PR layer and inner retina varied in different regions. The superior central retina, including the area centralis, was thickest (PR layer: 115-123 microm, entire retina: 225-230 microm, in fixed tissue). The peripheral retina was approximately 40% thinner than the center. Fresh retina was approximately 7.9% thicker than the fixed retina. Both vibratomy and excimer laser ablation removed the inner retina, leaving a PR-layer sheet with good morphology. To produce good quality PR sheets with vibratomy, use of different gelatin concentrations (2% to 35%) at various stages of sheet preparation was crucial. To produce PR sheets of uniform thickness with excimer laser ablation, control of fluid on the ret...Continue Reading

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Citations

Aug 5, 2011·Proceedings. Biological Sciences·Mandeep S Singh, Robert E MacLaren
Mar 3, 2007·The Journal of Clinical Investigation·Martin Friedlander
Jan 23, 2016·Trends in Molecular Medicine·Marco Zarbin
Jul 10, 2012·Progress in Retinal and Eye Research·Magdalene J Seiler, Robert B Aramant
Apr 27, 2011·Journal of Cellular Physiology·Teisha J RowlandDennis O Clegg
Jul 26, 2002·Experimental Eye Research·Robert B Aramant, Magdalene J Seiler
Sep 16, 1999·Experimental Neurology·A S KwanR D Lund
Nov 4, 2017·Neurotoxicity Research·Amy E Lindsey, Ellen Townes-Anderson
Feb 20, 2010·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Sara Royce Hynes, Erin B Lavik

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