PMID: 2114174Jun 20, 1990Paper

Preparation and utilization of fluorescent synthetic peptides

Biochimica Et Biophysica Acta
A ChersiA Nista

Abstract

In this study, several methods for controlled labelling of synthetic peptides by the use of fluorescent compounds (fluorescein isothiocyanate and dimethylaminonaphthalene sulfonyl chloride) were investigated. The first reagent yielded monofluoresceinated, active compounds only when the peptides lacked lysine residues. Monolabelling of peptides in solution with dimethylaminonaphthalenesulphonyl chloride was hindered by the broad reactivity of the reagent, but was achieved by reacting the fluorochrome on protected resin-bound peptides in solid-phase synthesis. The remarkable stability of the linkage allowed the cleavage of the peptide from the resin and deprotection of side-chain functions without hydrolysis of the labelled group. The binding of antipeptide antibodies to the labelled fragments was then estimated using different techniques.

References

Apr 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·T J PalkerD P Bolognesi
Dec 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·A R NeurathN Strick
Jan 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·W R ChurchR A Reisfeld

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Citations

Sep 8, 2001·Journal of Peptide Science : an Official Publication of the European Peptide Society·L FülöpM Zarándi
Sep 12, 2015·Journal of Peptide Science : an Official Publication of the European Peptide Society·Toshiaki HaraSaburo Aimoto
Jul 19, 1997·Biochimica Et Biophysica Acta·A ChersiL Rosanò

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