PMID: 6967514Aug 1, 1980Paper

Preparation, characterization, and immunogenicity of Haemophilus influenzae type b polysaccharide-protein conjugates

The Journal of Experimental Medicine
R SchneersonJ B Robbins

Abstract

A method is presented for covalently bonding Haemophilus influenzae type b capsular polysaccharide (HIB Ps) to several proteins. The method is efficient and relies upon the use of adipic dihydrazide as a spacer between the capsular polysaccharide and the carrier protein. In contrast to the poor immunogenicity of the purified HIB Ps in mice and rabbits, the HIB Ps-protein conjugates induced serum anti-type b antibodies having bactericidal activity at levels shown to be protective in humans when low doses were injected subcutaneously in a saline solution. The antibody response in mice was related to the dose of the conjugates, increased with the number of injections, and could be primed by the previous injection of the carrier protein. The HIB Ps-protein conjugates were immunogenic in three different mouse strains. The importance of the carrier molecule for the enhanced immunogenicity of the HIB Ps-protein conjugates was shown by the failure of HIB Ps hybrids prepared with either the homologous polysaccharide or pneumococcus type 3 polysaccharide to induce antibodie in mice. Rabbits injected with the HIB Ps-protein conjugates emulsified in Freund's adjuvant produced high levels of serum anti-type b antibodies which induced a bact...Continue Reading

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Citations

Jan 1, 1984·Infection·P H Mäkelä
Oct 10, 1985·Journal of Immunological Methods·A SuttonR Schneerson
Nov 25, 1988·Journal of Immunological Methods·A BarraJ L Preud'homme
Jun 2, 1990·Lancet·E R Moxon, R Rappuoli
Mar 18, 2004·Biologicals : Journal of the International Association of Biological Standardization·Sjoerd RijpkemaChristopher Jones
Sep 29, 2001·Journal of Microbiological Methods·G B Lesinski, M A Westerink
Oct 3, 1999·Vaccine·A A Lindberg
Jan 26, 2000·Comptes rendus de l'Académie des sciences. Série III, Sciences de la vie·A A Lindberg

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