Preparation, Characterization, and Pharmacokinetic Study of a Novel Long-Acting Targeted Paclitaxel Liposome with Antitumor Activity

International Journal of Nanomedicine
Bing HanJin Pei

Abstract

Breast cancer is the leading cause of cancer death in women. Chemotherapy to inhibit the proliferation of cancer cells is considered to be the most important therapeutic strategy. The development of long-circulating PEG and targeting liposomes is a major advance in drug delivery. However, the techniques used in liposome preparation mainly involve conventional liposomes, which have a short half-life, high concentrations in the liver and spleen reticuloendothelial system, and no active targeting. Four kinds of paclitaxel liposomes were prepared and characterized by various analytical techniques. The long-term targeting effect of liposomes was verified by fluorescence detection methods in vivo and in vitro. Pharmacokinetic and acute toxicity tests were conducted in ICR mice to evaluate the safety of different paclitaxel preparations. The antitumor activity of ES-SSL-PTX was investigated in detail using in vitro and in vivo human breast cancer MCF-7 cell models. ER-targeting liposomes had a particle size of 137.93±1.22 nm and an acceptable encapsulation efficiency of 88.07±1.25%. The liposome preparation is best stored at 4°C, and is stable for up to 48 hrs. Cytotoxicity test on MCF-7 cells demonstrated the stronger cytotoxic activ...Continue Reading

Citations

Sep 26, 2020·Frontiers in Molecular Biosciences·Yihan YaoAnwen Shao
Jun 5, 2020·International Journal of Nanomedicine·Yanhua JiangTe Fang
May 21, 2021·International Journal of Nanomedicine·Guo YuFakhar Ud Din

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Methods Mentioned

BETA
dynamic light scattering
Fluorescence microscopy
fluorescence imaging

Software Mentioned

GraphPad Prism

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