Preparation, fabrication and biocompatibility of novel injectable temperature-sensitive chitosan/glycerophosphate/collagen hydrogels.

Journal of Materials Science. Materials in Medicine
Kedong SongZhanfeng Cui

Abstract

This paper introduces a novel type of injectable temperature-sensitive chitosan/glycerophosphate/collagen (C/GP/Co) hydrogel that possesses great biocompatibility for the culture of adipose tissue-derived stem cells. The C/GP/Co hydrogel is prepared by mixing 2.2% (v/v) chitosan with 50% (w/w) β-glycerophosphate at different proportions and afterwards adding 2 mg/ml of collagen. The gelation time of the prepared solution at 37°C was found to be of around 12 min. The inner structure of the hydrogel presented a porous spongy structure, as observed by scanning electron microscopy. Moreover, the osmolality of the medium in contact with the hydrogel was in the range of 310-330 mmol kg(-1). These analyses have shown that the C/GP/Co hydrogels are structurally feasible for cell culture, while their biocompatibility was further examined. Human adipose tissue-derived stem cells (ADSCs) were seeded into the developed C/GP and C/GP/Co hydrogels (The ratios of C/GP and C/GP/Co were 5:1 and 5:1:6, respectively), and the cellular growth was periodically observed under an inverted microscope. The proliferation of ADSCs was detected using cck-8 kits, while cell apoptosis was determined by a Live/Dead Viability/Cytotoxicity kit. After 7 days of...Continue Reading

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Citations

Mar 26, 2013·Journal of Materials Science. Materials in Medicine·Ya Nan WeiXi Guang Chen
Jun 22, 2012·Applied Biochemistry and Biotechnology·Kedong SongLing Wang
Oct 8, 2013·Materials Science & Engineering. C, Materials for Biological Applications·Fereshteh MirahmadiShahin Bonakdar
Apr 5, 2016·Materials Science & Engineering. C, Materials for Biological Applications·Payam BaeiNasser Aghdami
May 9, 2015·Materials Science & Engineering. C, Materials for Biological Applications·Wen-Ta SuChih-Ming Chou
Feb 25, 2011·Acta Biomaterialia·Limin Wang, Jan P Stegemann
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Mar 29, 2020·Stem Cell Research & Therapy·Yong YaoZuo-Xiang He
Nov 28, 2012·Advanced Healthcare Materials·David J CaldwellJan P Stegemann
May 9, 2021·Acta Biomaterialia·Hongzhou PengXiaonong Zhang

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