Preparation of 2'-deoxy-2'-methylseleno-modified phosphoramidites and RNA

Current Protocols in Nucleic Acid Chemistry
Ronald MicuraHolger Moroder

Abstract

The derivatization of nucleic acids with selenium is a useful approach to facilitate phase determination during three-dimensional structural analysis by X-ray crystallography. This unit describes (1) the synthesis and characterization of 2'-deoxy-2'-methylseleno (2'-Se-methyl) nucleosides and their corresponding 3'-O-(2-cyanoethyl)-N,N-diisopropylphosphoramidite derivatives, (2) the site-specific incorporation of 2'-Se-methyl ribonucleosides into oligoribonucleotides by chemical RNA solid-phase synthesis, and (3) the enzymatic ligation of Se-containing RNA oligonucleotides to produce a biologically relevant RNA sequence.

References

Jan 5, 2002·Journal of the American Chemical Society·Quan DuZhen Huang
Jul 24, 2002·Acta Crystallographica. Section D, Biological Crystallography·E EnnifarP Dumas
Dec 12, 2002·Journal of the American Chemical Society·Christopher J WildsMartin Egli
Jan 30, 2004·Journal of the American Chemical Society·Claudia Höbartner, Ronald Micura
Jun 4, 2004·Nature·Peter L AdamsScott A Strobel
Feb 22, 2005·Nature Structural & Molecular Biology·Alexander SerganovDinshaw J Patel
Aug 25, 2005·Journal of the American Chemical Society·Claudia HöbartnerRonald Micura
Jul 27, 2006·Journal of the American Chemical Society·Holger MoroderRonald Micura

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