PMID: 6774356Sep 1, 1980Paper

Preparation of [3H]-12-L-hydroxyeicosatetraenoic acid and its use in radioimmunoassay

Prostaglandins and Medicine
J D EskraT J Carty

Abstract

An efficient method for the enzymic preparation of high specific radioactivity [3H]-12-L-hydroxyeicosatetraenoic acid (12-L-HETE) is described. This compound was used as a radiolabeled ligand in the radioimmunoassay (RIA) of 12-L-HETE. The accuracy of the RIA was checked by incubating [14C]arachidonic acid with platelet lipoxygenase, and measuring enzyme activity in the presence of the inhibitor, 1-phenyl-3-pyrazolidinone (phenidone). The amount of 12-L-HETE synthesized, determined by RIA, was found to be in agreement with that analyzed by radiochemical assay after thin layer plate chromatography.

References

Feb 20, 1975·Biochimica Et Biophysica Acta·D H Nugteren
Sep 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·M Hamberg, B Samuelsson
Dec 11, 1974·Biochemical and Biophysical Research Communications·M Hamberg, B Samuelsson
Oct 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·M HambergB Samuelsson
Jan 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·M I SiegelP Cuatrecasas

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