Preparation of a β-Cyclodextrin-Based Open-Tubular Capillary Electrochromatography Column and Application for Enantioseparations of Ten Basic Drugs

PloS One
Linlin FangXingjie Guo

Abstract

An open-tubular capillary electrochromatography column was prepared by chemically immobilized β-cyclodextrin modified gold nanoparticles onto new surface with the prederivatization of (3-mercaptopropyl)-trimethoxysilane. The synthesized nanoparticles and the prepared column were characterized by transmission electron microscopy, scanning electron microscopy, infrared spectroscopy and ultraviolet visible spectroscopy. When the column was employed as the chiral stationary phase, no enantioselectivity was observed for ten model basic drugs. So β-cyclodextrin was added to the background electrolyte as chiral additive to expect a possible synergistic effect occurring and resulting in a better separation. Fortunately, significant improvement in enantioselectivity was obtained for ten pairs of drug enantiomers. Then, the effects of β-cyclodextrin concentration and background electrolyte pH on the chiral separation were investigated. With the developed separation mode, all the enantiomers (except for venlafaxine) were baseline separated in resolutions of 4.49, 1.68, 1.88, 1.57, 2.52, 2.33, 3.24, 1.63 and 3.90 for zopiclone, chlorphenamine maleate, brompheniramine maleate, dioxopromethazine hydrochloride, carvedilol, homatropine hydrobr...Continue Reading

References

Aug 22, 2003·Journal of Chromatography. a·Thomas O'MahonyJeremy D Glennon
Nov 25, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Csilla Misl'anová, Milan Hutta
Dec 9, 2003·Electrophoresis·Constantina P Kapnissi-ChristodoulouIsiah M Warner
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Sep 13, 2011·Electrophoresis·Debby Mangelings, Yvan Vander Heyden

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Citations

Apr 21, 2020·Electrophoresis·Tong Ye WangSergey N Krylov
May 12, 2020·ChemPlusChem·Christopher HobbsMichal Řezanka
May 6, 2020·Biomedical Chromatography : BMC·Gabriel HancuEnikő Barabás-Hajdu
Aug 18, 2017·Electrophoresis·Faustino M TarongoyJoselito P Quirino
Oct 19, 2019·Analytica Chimica Acta·Mohamed Adel AhmedJoselito P Quirino

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Methods Mentioned

BETA
scanning electron microscopy
electrophoresis

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