PMID: 8581374Oct 1, 1995Paper

Preparation of a bispecific F(ab')2 targeted to the human IL-2 receptor

Journal of Hematotherapy
J Y TsoJ Hakimi

Abstract

The heterodimer-forming leucine zippers Fos and Jun can efficiently mediate the formation of bispecific F(ab')2 when they are fused separately to two different Fab' fragments. This recombinant method can be used in conjunction with the humanization process to yield humanized bispecific F(ab')2. The potential immunogenicity of the leucine zippers can be eliminated by their removal using pepsin digestion. This method has been scaled up to produce hundreds of milligrams of a bispecific F(ab')2 that targets two subunits of the human IL-2 receptor.

Citations

Jun 6, 2000·Advanced Drug Delivery Reviews· de Leij LB J Kroesen
Sep 2, 1998·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·S K Vaswani, R G Hamilton
Jun 1, 1997·Immunotechnology : an International Journal of Immunological Engineering·A Plückthun, P Pack
Oct 17, 2012·Analytical Biochemistry·Gregory M K Poon
Jan 18, 2005·Biochemical and Biophysical Research Communications·Bao-Cheng HuangStephen J Kennel
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