Preparation of a peptide vaccine against GnRH by a bioprocess system based on asparaginase

Vaccine
Xuejun WangJingjing Liu

Abstract

GnRH is a promising target in hormone-dependent cancer immunotherapy. In our previous study, we have designed and purified a peptide vaccine GhM (GnRH3-hinge-MVP) by use of the bioprocess system based on asparaginase. Active immunization with GhM in the presence of CFA/IFA evoked strong humoral response. In this study, the motif NRLLLTG with high affinity to nanoparticle carrier VLP HBcDelta-SBD was fused to the C terminus of GhM to form a new peptide vaccine GhMNR (GnRH3-hinge-MVP-NRLLLTG). The fusion protein ansB-C-GhMNR was controlled by vigorous T7lac promotor and expressed effectively as inclusion bodies after induction by lactose and then purified by means of cell disruption, washing and cold ethanol fractionation. After hydrolyzed for 72 h, GhMNR was liberated from the fusion partner ansB-C and purified by CM52 cation exchange chromatography. These results suggested that the bioprocess system is suitable for large-scale expression and purification of the peptide vaccine GhMNR, and even some other proteins or peptides which may be important for industrial or laboratory purposes.

References

Sep 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·C CarelliL Chedid
May 8, 1998·Journal of Biotechnology·A K Gombert, B V Kilikian
Mar 9, 2004·Protein Expression and Purification·Song-shan TangJing-jing Liu
Jan 7, 2005·Applied Microbiology and Biotechnology·S Jana, J K Deb
Mar 24, 2005·Journal of Molecular Biology·Simone PoppJochen Reinstein
May 27, 2008·Current Opinion in Immunology·Yoshihiro OkaHaruo Sugiyama
Nov 15, 2008·Trends in Endocrinology and Metabolism : TEM·Ilpo HuhtaniemiBo-Eric Persson
May 15, 2009·Proceedings of the National Academy of Sciences of the United States of America·Eric B BertelsenErik R P Zuiderweg

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Citations

Mar 7, 2014·Applied Microbiology and Biotechnology·Xue Jun WangRong Yue Cao
Aug 31, 2010·Cancer Immunology, Immunotherapy : CII·Xue Jun WangJing Jing Liu

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