Preparation of a promising angiogenesis PET imaging agent: 68Ga-labeled c(RGDyK)-isothiocyanatobenzyl-1,4,7-triazacyclononane-1,4,7-triacetic acid and feasibility studies in mice

Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine
Jae Min JeongMyung Chul Lee

Abstract

Arg-Gly-Asp (RGD) derivatives have been labeled with various radioisotopes for the imaging of angiogenesis in ischemic tissue, in which alpha(v)beta(3) integrin plays an important role. In this study, cyclic Arg-Gly-Asp-D-Tyr-Lys [c(RGDyK)] was conjugated with 2-(p-isothiocyanatobenzyl)-1,4,7-triazacyclononane-1,4,7-triacetic acid (SCN-Bz-NOTA) and then labeled with (68)Ga. The labeled RGD so produced was subjected to an in vitro binding assay and in vivo biodistribution and PET studies. A mixture of SCN-Bz-NOTA (660 nmol) and c(RGDyK) (600 nmol) in 0.1 M sodium carbonate buffer (pH 9.5) was allowed to react for 20 h at room temperature in the dark for thiourea bond formation. The conjugate obtained was purified by semipreparative high-performance liquid chromatography (HPLC). The purified c(RGDyK)-SCN-Bz-NOTA (NOTA-RGD) was then labeled with (68)Ga from a (68)Ge/(68)Ga generator and purified by semipreparative HPLC. A competitive binding assay for c(RGDyK) and NOTA-RGD was performed with (125)I-c(RGDyK) as a radioligand and alpha(v)beta(3) integrin-coated plates as a solid phase. (68)Ga-NOTA-RGD (0.222 MBq/100 microL) was injected, through a tail vein, into mice with hind limb ischemia and into mice bearing human colon cancer ...Continue Reading

References

Dec 15, 1992·European Journal of Biochemistry·M GurrathR Timpl
Jan 1, 1987·Methods in Enzymology·R PytelaE Ruoslahti
Sep 23, 2000·Nature·P Carmeliet, R K Jain
Jan 23, 2003·Cancer Biotherapy & Radiopharmaceuticals·Marcel JanssenOtto C Boerman
Jan 22, 2004·Bioconjugate Chemistry·Roland HaubnerMarkus Schwaiger
Mar 12, 2004·Nuclear Medicine and Biology·Xiaoyuan ChenPeter S Conti
Jul 18, 2006·Nuclear Medicine and Biology·Yun-Sang LeeYoung-Ger Suh
Mar 3, 2007·European Journal of Nuclear Medicine and Molecular Imaging·Wouter A P Breeman, Alfons M Verbruggen
Jun 19, 2007·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Zi-Bo LiXiaoyuan Chen
Sep 18, 2007·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Konstantin P ZhernosekovFrank Rösch

❮ Previous
Next ❯

Citations

Jul 25, 2009·European Journal of Nuclear Medicine and Molecular Imaging· Johanna HaukkalaAnne Roivainen
Jun 19, 2010·European Journal of Nuclear Medicine and Molecular Imaging·Roland HaubnerXiaoyuan Chen
Mar 6, 2012·European Journal of Nuclear Medicine and Molecular Imaging·Melpomeni Fani, Helmut R Maecke
Mar 6, 2012·European Journal of Nuclear Medicine and Molecular Imaging·F C GaertnerA J Beer
Jan 26, 2012·European Journal of Nuclear Medicine and Molecular Imaging·Haokao GaoXiaoyuan Chen
Oct 26, 2012·European Journal of Nuclear Medicine and Molecular Imaging·Choong Mo KangYearn Seong Choe
May 16, 2013·European Journal of Nuclear Medicine and Molecular Imaging·Luca MenichettiLeonardo Manzoni
Mar 23, 2012·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Ning GuoXiaoyuan Chen
Aug 7, 2013·Annals of Nuclear Medicine·Jin Chul PaengDong Soo Lee
Dec 13, 2012·Applied Radiation and Isotopes : Including Data, Instrumentation and Methods for Use in Agriculture, Industry and Medicine·Daniel L SmithJennifer Sims-Mourtada
Mar 26, 2013·Applied Radiation and Isotopes : Including Data, Instrumentation and Methods for Use in Agriculture, Industry and Medicine·Sangeeta Ray Banerjee, Martin G Pomper
Sep 10, 2011·Inorganic Chemistry·Jean-François Morfin, Éva Tóth
May 16, 2012·Inorganic Chemistry·Eszter BorosChris Orvig
Mar 29, 2011·Dalton Transactions : an International Journal of Inorganic Chemistry·Brian M Zeglis, Jason S Lewis
Sep 17, 2011·Metallomics : Integrated Biometal Science·Daniela KretschyStephan Hann
May 5, 2009·Cancer Biotherapy & Radiopharmaceuticals·Valentina AmbrosiniDomenico Rubello
Jan 6, 2011·Cancer Biotherapy & Radiopharmaceuticals·Chang Hwan JuSeo Young Jeong
Jun 12, 2012·International Journal of Molecular Imaging·Dinesh ShettyHyunsuk Shim
Apr 5, 2013·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Weixing WanXiaoyuan Chen
Oct 13, 2011·Nuklearmedizin. Nuclear Medicine·F BucheggerJ O Prior
Jan 8, 2014·Theranostics·Irina Velikyan
Aug 29, 2013·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Jinxia GuoXiaoyuan Chen
Feb 28, 2014·International Journal of Molecular Imaging·Alejandro Amor-CoarasaAnthony J McGoron
Apr 8, 2014·Journal of Pharmaceutical and Biomedical Analysis·Nathalie BrackeBart De Spiegeleer
Apr 14, 2011·European Journal of Nuclear Medicine and Molecular Imaging·Peter A KnetschRoland Haubner
Dec 1, 2010·Nuclear Medicine and Molecular Imaging·Dinesh ShettyJae Min Jeong
Mar 1, 2011·Nuclear Medicine and Molecular Imaging·Aizhi Zhu, Hyunsuk Shim
Jan 8, 2016·Nanomedicine : Nanotechnology, Biology, and Medicine·Sung-Hyun MoonJae Min Jeong
Nov 9, 2010·Nuclear Medicine and Biology·Dinesh ShettyMyung Chul Lee
Jul 15, 2015·European Journal of Nuclear Medicine and Molecular Imaging·Song GaoShuanghu Yuan
Jul 29, 2015·Applied Radiation and Isotopes : Including Data, Instrumentation and Methods for Use in Agriculture, Industry and Medicine·Si-Yang WangJian-Ping Zhang
Jul 23, 2011·Journal of Magnetic Resonance Imaging : JMRI·Guoqiu WuNaifeng Liu
Dec 14, 2011·Contrast Media & Molecular Imaging·Ute MühlhausenTobias Bäuerle

❮ Previous
Next ❯

Related Concepts

Related Feeds

Brain Ischemia

Brain ischemia is a condition in which there is insufficient blood flow to the brain to meet metabolic demand. Discover the latest research on brain ischemia here.

Related Papers

European Journal of Nuclear Medicine and Molecular Imaging
Zi-Bo LiXiaoyuan Chen
European Journal of Nuclear Medicine and Molecular Imaging
Peter A KnetschRoland Haubner
European Journal of Nuclear Medicine and Molecular Imaging
Clemens DecristoforoRoland Haubner
© 2022 Meta ULC. All rights reserved