PMID: 2488739Jan 1, 1989Paper

Preparation of a protein mucin solution to be used in patients with xerostomia (Venezuela)

Acta odontológica venezolana
L A EscalonaA M Acevedo

Abstract

The purpose of this study was to prepare a protein solution to simulate natural saliva using mucin extracted from bovine submaxillary gland. The extraction process was done in keeping with Drans & Leung's (1980) modified technique. Once the bovine submaxillary gland was extracted (approximately 500 g), it is cut and stored in distilled water for 12 hrs at 4 degrees C in order to eliminate any tissue or blood remaining in the section. The tissue is then homogenized and suspended in a phosphate buffer solution with a pH of 7 for 48 hours. The solution is then centrifuged and the supernatant is treated to extract the protein present in the solution. The remaining solution is dialyzed with distilled water, changed three times and then lyophilized. The lyophilized product is diluted in distilled water (1 g/1) taking aliquots to determine the pureness of the proteins. The solution obtained has a viscosity and protein composition which is very similar and offers great advantages for use in patients who suffer from severe xerostomy.

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.