Preparation of abiotic polymer nanoparticles for sequestration and neutralization of a target peptide toxin

Nature Protocols
Keiichi YoshimatsuKenneth J Shea

Abstract

Synthetic polymer nanoparticles (NPs) with intrinsic affinity for target biomacromolecules hold great promise in the development of novel tools for biological and biomedical research. We recently reported the design and synthesis of abiotic, synthetic polymer NPs with high intrinsic affinity for a peptide toxin melittin. The NP was selected by screening a small library of NPs (∼100 nm) composed of various ratios of monomers that contain functional groups complementary to the peptide melittin. The selected polymer NP, a co-polymer of acrylic acid (AAc), N-tert-butylacrylamide (TBAm), N-isopropylacrylamide (NIPAm) and N,N'-methylenebisacrylamide (BIS), effectively captures and neutralizes the toxicity of the peptide through a combination of electrostatic and hydrophobic interactions. This protocol describes a step-by-step procedure for the preparation and evaluation of synthetic polymer NPs for sequestration and neutralization of the target peptide toxin. The polymer NPs can be synthesized in a one-step polymerization reaction using commercially available reagents. The polymerization reaction for the synthesis of polymer NPs takes several hours, and the total protocol including subsequent purification and characterization by dyna...Continue Reading

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Citations

Feb 13, 2016·Nature Protocols·Francesco CanfarottaSergey Piletsky
Feb 27, 2016·Critical Reviews in Oncology/hematology·Valentina FlaminiYu-Xin Cui
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Dec 11, 2020·Journal of Controlled Release : Official Journal of the Controlled Release Society·Adrija JhaSrinivas Mutalik
Mar 31, 2021·Biomacromolecules·Keiichi YoshimatsuKenneth J Shea
Sep 14, 2019·Biomacromolecules·Hiroyuki KoideKenneth J Shea
Nov 17, 2017·Langmuir : the ACS Journal of Surfaces and Colloids·Ajith PattammattelChalla V Kumar

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