Preparation of albumin based nanoparticles for delivery of fisetin and evaluation of its cytotoxic activity

International Journal of Biological Macromolecules
Pooja GhoshSwagata Dasgupta

Abstract

Fisetin is a well known flavonoid that shows several properties such as antioxidant, antiviral and anticancer activities. Its use in the pharmaceutical field is limited due to its poor aqueous solubility which results in poor bioavailability and poor permeability. The aim of our present study is to prepare fisetin loaded human serum albumin nanoparticles to improve its bioavailability. The nanoparticles were prepared by a desolvation method and characterized by spectroscopic and microscopic techniques. The particles were smooth and spherical in nature with an average size of 220 ± 8 nm. The encapsulation efficiency was found to be 84%. The in vitro release profile showed a biphasic pattern and the release rate increases with increase in ionic strength of solution. We have also confirmed the antioxidant activity of the prepared nanoparticles by a DPPH (2,2-diphenyl-1-picrylhydrazyl) assay. Further its anticancer activity was evaluated using MCF-7 breast cancer cell lines. Our findings suggest that fisetin loaded HSA nanoparticles could be used to transfer fisetin to target areas under specific conditions and thus may find use as a delivery vehicle for the flavonoid.

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Citations

May 25, 2016·Expert Opinion on Drug Delivery·Mahdi KarimiMichael R Hamblin
Sep 6, 2020·Biomolecules·Malgorzata DobrzynskaEwa Florek
Aug 3, 2019·Seminars in Cancer Biology·Haroon KhanMohammad Amjad Kamal
Jun 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Wan-Yi LiuYu-Tse Wu
Jul 25, 2021·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Ammad Ahmad FarooqiBaojun Xu
Aug 19, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Piyush MehtaC Bothiraja

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