Preparation of an open-tubular capillary column with a monolithic layer of S-ketoprofen imprinted and 4-styrenesulfonic acid incorporated polymer and its enhanced chiral separation performance in capillary electrochromatography

Journal of Chromatography. a
Shabi Abbas Zaidi, Won Jo Cheong

Abstract

Enhanced chiral separation performance has been observed for ketoprofen enantiomers in capillary electrochromatography (CEC) with an open-tubular (OT) column prepared with a specific molecule imprinted polymer (MIP) on the innerwall of 50mum ID capillary. The column was prepared by in situ thermal polymerization inside the pretreated and silanized fused silica capillary. A specific diluted monomer mixture composed of S-ketoprofen, methacrylic acid (MAA, functional monomer), ethylene glycol dimethacrylate (EDMA, cross-linker), and 4-styrenesulfonic acid (4-SSA) dissolved in 9/1 (v/v) acetonitrile/2-propanol was used to fabricate the OT-MIP layer. 4-SSA was added to form a MIP layer capable of stable and strong electro-osmotic flow (EOF) over the pH range of this study securing CEC elution of ketoprofen having partial negative charge near the optimized pH. Various parameters such as buffer pH, organic modifier composition, salt concentration, and applied potential have been optimized for CEC chiral separation of ketoprofen enantiomers. Very good separation selectivity and efficiency were observed, thus the chromatographic resolution of ketoprofen enantiomers was as high as 10.5, and the number of theoretical plates of R-ketoprofe...Continue Reading

References

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Citations

Oct 18, 2016·Drug Delivery·Shabi Abbas Zaidi
Apr 6, 2012·Journal of Chromatography. a·Ze-Hui WeiZhao-Sheng Liu
Jun 29, 2010·Journal of Pharmaceutical and Biomedical Analysis·Michael Lämmerhofer, Andrea Gargano
Sep 13, 2011·Electrophoresis·Debby Mangelings, Yvan Vander Heyden
Jan 3, 2013·Journal of Separation Science·Won Jo CheongFaiz Ali
Jul 22, 2014·Electrophoresis·Bogdan-Cezar IacobRadu Oprean
Apr 5, 2014·Journal of Molecular Recognition : JMR·Michael J WhitcombeIan A Nicholls
Dec 17, 2014·Electrophoresis·Li-Na MuZhao-Sheng Liu
Feb 11, 2015·Journal of Colloid and Interface Science·M MonierHossam M Nassef
Jan 3, 2019·Critical Reviews in Analytical Chemistry·Shabi Abbas Zaidi
Mar 1, 2011·Analytical Methods : Advancing Methods and Applications·Huang Lu, Guonan Chen
Jan 23, 2021·Journal of Separation Science·Thomas BouvarelValérie Pichon
May 26, 2010·Analytical Chemistry·Timothy J Ward, Karen D Ward
Apr 13, 2011·Analytical Chemistry·R K Gilpin, C S Gilpin

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