PMID: 8066995May 1, 1994

Preparation of anti-canine serum amyloid A (SAA) serum and purification of SAA from canine high-density lipoprotein

Veterinary Immunology and Immunopathology
S YamamotoY Rikihisa

Abstract

Antiserum to canine serum amyloid A (SAA) was prepared in rabbits by immunization with crude SAA which was prepared from high-density lipoprotein 3 (HDL3) obtained from canine acute-phase serum. The antiserum was absorbed for contaminating antibodies by affinity chromatography using Sepharose 4B coupled with normal canine serum proteins. The rabbit anti-canine SAA serum reacted with a protein and formed a single precipitin line at the position of the alpha 1-region of the immunoelectrophoresis of canine acute-phase serum but did not react with the normal canine serum on immunoelectrophoresis. The antibody to canine SAA was also confirmed by Western blotting analysis. Canine SAA was purified as a low molecular weight protein component from crude SAA by preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) after gel filtration chromatography. Purified canine SAA had a molecular weight of 15,000 as estimated by SDS-PAGE. This SAA level was found by enzyme-linked immunosorbent assay (ELISA) to increase 1 day after inoculation with Bordetella bronchiseptica to 9.0-20.1 times the preinoculation value.

References

Apr 1, 1975·The Journal of Clinical Investigation·C J Rosenthal, E C Franklin
Jan 1, 1977·Scandinavian Journal of Immunology·P M JohnsonE Linder
Jan 1, 1978·Scandinavian Journal of Immunology·J GoudswaardJ P Vaerman
Sep 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·E P Benditt, N Eriksen
Jan 1, 1979·Scandinavian Journal of Immunology·B SkogenT E Michaelsen
Aug 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·E P BendittR H Hanson
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Feb 1, 1978·The Journal of Clinical Investigation·K P McAdamS M Wolff
Jan 31, 1992·Veterinary Immunology and Immunopathology·S YamamotoM Naiki
Jan 24, 1991·Journal of Immunological Methods·Y S Taktak, M Lee
Apr 1, 1974·The Journal of Clinical Investigation·G Husby, J B Natvig
Nov 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·N Eriksen, E P Benditt
Jul 16, 1984·Clinica Chimica Acta; International Journal of Clinical Chemistry·N Eriksen, E P Benditt
Nov 1, 1983·Acta paediatrica Scandinavica·G MarhaugG Husby

Citations

Mar 28, 2008·Veterinary Clinical Pathology·Alexandre MerloSílvia Regina Ricci Lucas

Related Concepts

Acute-Phase Reaction
Amyloid Protein AA
Metazoa
Bordetella Infections
Chromatography, Affinity
Molecular Sieve Chromatography
Dog Diseases
Canis familiaris
SDS-PAGE
Immunoelectrophoresis

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