Preparation of anti-human thymidine phosphorylase monoclonal antibodies useful for detecting the enzyme levels in tumor tissues

Biological & Pharmaceutical Bulletin
M NishidaH Ishitsuka

Abstract

The antitumor activity of cytostatic 5'-deoxy-5-fluorouridine (5'-dFUrd) depends on its being converted to 5-fluorouracil (5-FUra) by the enzyme thymidine phosphorylase (dThdPase, EC 2.42.4). We prepared mouse anti-human dThdPase monoclonal antibodies to serve as tools for clinical studies with this drug. Partially purified dThdPase obtained form HCT116 human colon cancer cells grown in athymic mice was used as and antigen for the immunization of mice. Six hybridomas were cloned which produced anti-human dThdPase antibodies, as detected by Western blot analysis with human dThdPase. With these antibodies, we developed an ELISA method sensitive enough to measure dThdPase levels, even in tumor tissue samples weighing as little as 10 mg. In addition, one monoclonal antibody was suitable for immunologically staining the enzyme in tumor tissues. Thus, these anti-human dThdPase monoclonal antibodies could be used to measure levels of the enzyme in tumor cells, which is essential for the activation of 5'-dFUrd.

Citations

Dec 4, 2002·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·M TerashimaK Saito
Mar 31, 2000·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·S YamamotoG Kajiyama
Mar 28, 2002·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·T NagataH Yamaue
Mar 19, 2004·European Journal of Surgical Oncology : the Journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology·T TsujiY Tagawa
May 23, 2002·Breast Cancer : the Journal of the Japanese Breast Cancer Society·Hirotoshi UtsunomiyaKennichi Kakudo
Apr 25, 2003·Journal of Biomedical Science·Jiro FujimotoTeruhiko Tamaya
Oct 3, 2006·Journal of Biomedical Science·Jiro FujimotoTeruhiko Tamaya
Apr 7, 2012·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Kiyoshi HasegawaYasuhiro Udagawa
Apr 15, 2008·Vascular Pharmacology·Vummidi Giridhar PremkumarPanchanatham Sachdanandam
Jun 16, 2004·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Tomohiro NishinaYasushi Shiratori
Nov 1, 2005·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Junichi KodamaYuji Hiramatsu
Apr 3, 2002·Japanese Journal of Cancer Research : Gann·Hidetoshi KinsuiHaruo Ito
Oct 16, 2002·Annals of Oncology : Official Journal of the European Society for Medical Oncology·J FujimotoT Tamaya
Oct 16, 2002·Annals of Oncology : Official Journal of the European Society for Medical Oncology·J FujimotoT Tamaya
Mar 7, 2013·Annals of Oncology : Official Journal of the European Society for Medical Oncology·V J HarveyM P Findlay
Jun 13, 2002·Diseases of the Esophagus : Official Journal of the International Society for Diseases of the Esophagus·N KoideJ Amano
Nov 7, 2003·Diseases of the Esophagus : Official Journal of the International Society for Diseases of the Esophagus·N KoideJ Amano
Dec 4, 2003·Diseases of the Esophagus : Official Journal of the International Society for Diseases of the Esophagus·T NoguchiH E Gabbert
Jun 1, 2004·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Jim CassidyEduardo Díaz-Rubio
Nov 30, 2005·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·M Wasif SaifMartin R Johnson
Dec 20, 2005·Cancer Chemotherapy and Pharmacology·Kaori F OuchiYutaka Tanaka
Dec 19, 2000·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·T KonoS Kasai
Nov 14, 1997·International Journal of Cancer. Journal International Du Cancer·K MaedaM Sowa

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.