Preparation of artificial ceroid/lipofuscin by UV-oxidation of subcellular organelles

Mechanisms of Ageing and Development
E Nilsson, D Yin

Abstract

Recent studies have consistently shown that, during oxidative damage, glycation, and other oxygen stress-related reactions, various biomolecules are converted into ceroid- and lipofuscin-like fluorescent pigments. In this study, artificial ceroid/lipofuscin was produced by exposing rat liver fractions to UV-light overnight. Thiobarbituric acid reactive substances (TBARS) were formed in increasing amounts during the early stages of the process, but decreased as the material was later converted into a polymeric structure with few remaining peroxides. In the transmission electron microscope the artificial pigment showed lamellar structures and was osmiophilic. By energy-dispersive X-ray analysis the material was found to contain Ca and Fe in the same way as natural ceroid/lipofuscin. Moreover, it exhibited ceroid/lipofuscin-like, greenish-yellowish autofluorescence when assayed by microfluorometry, with a fluorescence maximum consistently found at 430 nm when excited at 350 nm. Identical fluorescence maxima were found for each fraction of rat liver that was used as the origin of the pigments, i.e. nuclei, mitochondria, lysosomes and microsomes. Extracts with either chloroform-methanol, or sodium dodecylsulphate, showed identical c...Continue Reading

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Citations

Jun 24, 2008·Experimental Aging Research·Xinhui WangShuli Sheng
Oct 16, 2010·Photochemistry and Photobiology·Sarah D LamoreGeorg T Wondrak
Jul 8, 1998·Proceedings of the National Academy of Sciences of the United States of America·L TsaiL I Szweda
Apr 8, 2014·Proceedings of the National Academy of Sciences of the United States of America·Marcelo M NociariEnrique Rodriguez-Boulan
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Sep 17, 2020·International Journal of Molecular Sciences·Eloise KeelingJ Arjuna Ratnayaka
Jul 22, 2018·Frontiers in Neuroscience·Alexandra Moreno-GarcíaMiguel Calero

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