Preparation of artificial skeletal muscle tissues by a magnetic force-based tissue engineering technique

Journal of Bioscience and Bioengineering
Yasunori YamamotoMasamichi Kamihira

Abstract

Artificial muscle tissues composed of mouse myoblast C2C12 cells were prepared using a magnetic force-based tissue engineering technique. C2C12 cells labeled with magnetite nanoparticles were seeded into the wells of 24-well ultralow-attachment culture plates. When a magnet was positioned underneath each plate, the cells accumulated evenly on the culture surface and formed multilayered cell sheets. Since the shapes of artificial tissue constructs can be controlled by magnetic force, cellular string-like assemblies were formed by using a linear magnetic field concentrator with a magnet. However, the resulting cellular sheets and strings shrank considerably and did not retain their shapes during additional culture periods for myogenic differentiation. On the other hand, when a silicone plug was positioned at the center of the well during the fabrication of a cell sheet, the cell sheet shrank drastically and formed a ring-like assembly around the plug. A histological examination revealed that the cells in the cellular ring were highly oriented in the direction of the circumference by the tension generated within the structure. Individual cellular rings were hooked around two pins separated by 10 mm, and successfully cultured for 6...Continue Reading

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Citations

Dec 7, 2013·Archives of Pharmacal Research·Eunjee A LeeNathaniel S Hwang
Jan 10, 2012·Tissue Engineering. Part C, Methods·Shawn P GroganDarryl D D'Lima
Dec 15, 2010·International Journal of Molecular Sciences·Hirokazu AkiyamaMasamichi Kamihira
Dec 11, 2013·Tissue Engineering. Part B, Reviews·Serge OstrovidovAli Khademhosseini
Oct 3, 2012·Journal of Bioscience and Bioengineering·Kazunori ShimizuEiji Nagamori
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Dec 10, 2013·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Rebecca M Duffy, Adam W Feinberg
Jun 3, 2015·Journal of Tissue Engineering and Regenerative Medicine·Kazushi IkedaMasamichi Kamihira
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Sep 14, 2016·Journal of Bioscience and Bioengineering·Tomohiro NakamuraToshia Fujisato
Mar 17, 2017·Scientific Reports·Kazushi IkedaMasamichi Kamihira
May 26, 2018·Biomedical Materials·Ana I GonçalvesManuela E Gomes
Sep 18, 2018·Tissue Engineering. Part a·Md ArifuzzamanMasamichi Kamihira
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Nov 14, 2021·Advanced Materials·Mohamadmahdi SamandariAli Tamayol

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