Preparation of bioactive and antimicrobial PLGA membranes by magainin II/EGF functionalization

International Journal of Biological Macromolecules
Emre YükselMenemşe Gümüşderelioğlu

Abstract

Development of dual functional materials that are capable of both reducing bacterial interaction and encouraging host tissue integration has gained importance in design of biomaterials. In this study, we prepared a bilayer poly (lactide co-glycolide) fibrous membrane with antibacterial and bioactive properties by electrospinning. The antibacterial layer was produced by covalent immobilization of antimicrobial peptide, Magainin II. The bioactive layer incorporating epidermal growth factor (EGF) molecules was subsequently electrospun on the antibacterial layer. The membranes were characterized by X-ray photoelectron spectroscopy, scanning electron microscopy and fluorescence microscopy. EGF release was detected by enzyme-linked immunosorbent assay. The antibacterial activity was tested against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). The ability to support tissue cell integration was detected by using L-929 mouse fibroblasts. The dual functional membranes established enhanced antibacterial properties and increased tissue cell compatibility. This combined approach suggests a promising strategy for wound dressings, vascular grafts and dental membranes as well as catheters and fixation devices.

References

Jun 28, 2008·The Surgeon : Journal of the Royal Colleges of Surgeons of Edinburgh and Ireland·J HardwickeD Thomas
Dec 11, 2014·Materials Science & Engineering. C, Materials for Biological Applications·Emre Yüksel, Ayşe Karakeçili

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Citations

Feb 6, 2017·Advances in Colloid and Interface Science·Randi Nordström, Martin Malmsten
Nov 24, 2018·Advanced Healthcare Materials·Carlos Mas-MorunoMatthew J Dalby
Jul 25, 2019·Macromolecular Bioscience·Alexander DartPeter Kingshott
Dec 31, 2019·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Morteza AbazariYaser Maleki
Jun 21, 2018·Advanced Healthcare Materials·Wei ShenXuesi Chen
Sep 4, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Xinyu SongZhiyu He

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