Preparation of core-shell magnetic polydopamine/alginate biocomposite for Candida rugosa lipase immobilization

Colloids and Surfaces. B, Biointerfaces
Chen HouHao Zhu

Abstract

A flexible, biocompatible and bioadhesive enzyme immobilizing material, which was synthesized based on the covalent assembly of biomimetic polymer and oxidized polysaccharide on magnetic nanoparticles (NPs), has been developed in this feasibility study. In this work, the bio-inspired polymer, polydopamine (PDA), was used to modify the well-monodispersed Fe3O4 NPs (mPDA NPs) with a controllable thickness via a dip-coating process, then the alginate di-aldehyde (ADA) was covalently assembled on the mPDA NPs and employed as a naturally occurring linking agent for Candida rugosa lipase (CRL) immobilization. The resulting support material was characterized by means of the transmission electron microscope (TEM), Fourier transform infrared spectra (FT-IR), X-ray diffraction (XRD), thermogravimetry (TG) analyser, and vibrating sample magnetometer (VSM). It was verified that the prepared mPDA NPs possessed distinct core-shell structure with uniform size and high saturation magnetization. For further application, the mPDA NPs was utilized in CRL immobilizing procedures and demonstrated can facilitate improving the enzyme activities. The optimum amount of lipase was 200 mg g(-1) support, the optimal pH and temperature for the catalyse con...Continue Reading

Citations

Dec 25, 2015·Colloids and Surfaces. B, Biointerfaces·Hua-jian YouYao-guang Zhang
Oct 19, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Fabian B H RehmBernd H A Rehm
Feb 25, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Yifu FuTianzhu Zhang
Aug 4, 2019·Applied Microbiology and Biotechnology·Danielle Gonçalves FilhoCarla Zanella Guidini
May 16, 2020·Materials Science & Engineering. C, Materials for Biological Applications·Xinli LiYanbao Zhao
Jul 6, 2021·Colloids and Surfaces. B, Biointerfaces·Hongbo SuoLili Xu

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