PMID: 943178Feb 10, 1976Paper

Preparation of cross-linked dimers of pancreatic ribonuclease

D WangS Moore


The cross-linking reaction between diimido esters and ribonuclease has been studied in terms of the yield of cross-linked dimer with optimum activity toward double-stranded RNA. With dimethyl suberimidate the most satisfactory conditions were condensation for 15 min at pH 7.5-8.0 at 21 degrees C with 1.25 mol equiv of the diimido ester and a protein concentration of 6%. The dimer (yield 20%) had 19 unmodified NH2 groups out of a theoretical 20 for a molecule in which two such groups are involved in the cross-linkage; the activity toward poly(A)-poly(U) in 0.14 M salt solution by spectrophotometric assay was 8.5 times that of the monomeric enzyme toward the same substrate.


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Nov 1, 1972·FEBS Letters·G D'AlessioE Leone
Jul 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·G E Davies, G R Stark
Mar 27, 1972·European Journal of Biochemistry·G D'AlessioE Leone
Jan 1, 1972·Methods in Enzymology·M J Hunter, M L Ludwig

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