PMID: 943178Feb 10, 1976Paper

Preparation of cross-linked dimers of pancreatic ribonuclease

Biochemistry
D WangS Moore

Abstract

The cross-linking reaction between diimido esters and ribonuclease has been studied in terms of the yield of cross-linked dimer with optimum activity toward double-stranded RNA. With dimethyl suberimidate the most satisfactory conditions were condensation for 15 min at pH 7.5-8.0 at 21 degrees C with 1.25 mol equiv of the diimido ester and a protein concentration of 6%. The dimer (yield 20%) had 19 unmodified NH2 groups out of a theoretical 20 for a molecule in which two such groups are involved in the cross-linkage; the activity toward poly(A)-poly(U) in 0.14 M salt solution by spectrophotometric assay was 8.5 times that of the monomeric enzyme toward the same substrate.

References

Aug 6, 1975·Biochimica Et Biophysica Acta·M LibonatiG D'Alessio
Mar 1, 1969·European Journal of Biochemistry·M Libonati, A Floridi
Nov 1, 1972·FEBS Letters·G D'AlessioE Leone
Jul 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·G E Davies, G R Stark
Mar 27, 1972·European Journal of Biochemistry·G D'AlessioE Leone
Jan 1, 1972·Methods in Enzymology·M J Hunter, M L Ludwig

❮ Previous
Next ❯

Citations

Sep 20, 1977·Journal of Molecular Evolution·J J BeintemaW M Fitch
Aug 26, 1980·Biochimica Et Biophysica Acta·S SorrentinoM Libonati
Nov 1, 1992·Enzyme and Microbial Technology·S S Wong, L J Wong
Nov 1, 1992·Enzyme and Microbial Technology·D E AlbertM H Keyes
Jul 24, 1998·Biochimica Et Biophysica Acta·G Gotte, M Libonati
Aug 14, 2010·Bioconjugate Chemistry·Thomas J RutkoskiRonald T Raines
May 5, 2010·DNA and Cell Biology·Marina PiscopoGiuseppe Geraci
May 1, 1982·European Journal of Biochemistry·S SorrentinoM Libonati
Nov 5, 2013·International Journal of Biological Macromolecules·Amit Kumar DindaSwagata Dasgupta
Jun 20, 1988·FEBS Letters·S A Benner
Oct 18, 2006·Proteins·Brigitte L SimonsMary Alice Hefford
Jan 1, 1979·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·J Bartholeyns, A Zenebergh
Jan 1, 1987·Archives of Biochemistry and Biophysics·M H PennerR E Feeney
Nov 27, 1979·Biochemistry·K F GeogheganR E Feeney
Mar 1, 1981·International Journal of Peptide and Protein Research·K F GeogheganR E Feeney
Jan 1, 1977·International Journal of Peptide and Protein Research·J BartholeynsW H Stein
Dec 19, 2019·Frontiers in Immunology·Giovanni Gotte, Marta Menegazzi
Jul 1, 1985·International Journal of Peptide and Protein Research·W S WongR E Feeney
Mar 6, 2010·Bioconjugate Chemistry·Francesca VottarielloMassimo Libonati

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
J Bartholeyns, P Baudhuin
European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)
J Bartholeyns, A Zenebergh
© 2021 Meta ULC. All rights reserved