Preparation of cryogel columns for depletion of hemoglobin from human blood

Artificial Cells, Nanomedicine, and Biotechnology
Ali DerazshamshirAdil Denizli

Abstract

In this study, we aimed to prepare the metal chelate affinity cryogels for the hemoglobin (Hb) depletion. Poly(2-hydroxyethyl methacrylate) (PHEMA) cryogels were selected as base matrix because of their blood compatibility, osmotic, chemical, and mechanical stability. Cryogels are also useful when working with the viscous samples such as blood, because of their interconnected macroporous structure. Iminodiacetic acid (IDA), the chelating agent, was covalently coupled with PHEMA cryogels after activation with the epichlorohydrin and then the Ni(II) ions were chelated to the IDA-bound cryogels. The depletion of the Hb from hemolysate was shown by SDS-PAGE.

References

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Jan 24, 2012·Colloids and Surfaces. B, Biointerfaces·Duygu Cimen, Adil Denizli
Sep 6, 2012·Artificial Cells, Nanomedicine, and Biotechnology·Işık PerçinAdil Denizli
Aug 10, 2013·Colloids and Surfaces. B, Biointerfaces·Lokman UzunAdil Denizli
Apr 2, 2014·Artificial Cells, Nanomedicine, and Biotechnology·Gözde Baydemir, Adil Denizli
Jul 22, 2014·Journal of Molecular Recognition : JMR·Gözde BaydemirAdil Denizli
Apr 14, 2013·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Adem YildirimMehmet Bayindir

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Citations

Sep 30, 2016·Artificial Cells, Nanomedicine, and Biotechnology·Kadir Erol
Oct 26, 2016·Artificial Cells, Nanomedicine, and Biotechnology·Kemal ÇetinAdil Denizli
Feb 20, 2018·Artificial Cells, Nanomedicine, and Biotechnology·Monireh BakhshpourAdil Denizli

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