Preparation of DNA from fixed, paraffin-embedded tissue

Current Protocols in Human Genetics
E A Fox

Abstract

Purification of DNA from fixed, paraffin-embedded tissue (PET) requires the removal of paraffin, proteins, and, in some cases, heavy metal ions. The resulting DNA is not generally suitable for Southern blot analysis. However, the polymerase chain reaction (PCR) can be used to amplify short regions of this DNA. The Basic Protocol is rapid and involves only a small number of manipulations, thus minimizing the possibility of contamination. The Alternate Protocol involves additional steps including extractions with xylenes and phenol/chloroform. This procedure is recommended as the primary approach when tissue has not been fixed in an optimal manner or as a secondary approach when the Basic Protocol has failed to produce satisfactory DNA.

References

Jun 1, 1990·Journal of Clinical Pathology·D P JacksonP Quirke
Jan 1, 1973·Progress in Nucleic Acid Research and Molecular Biology·M Y Feldman

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