PMID: 6402547Jan 28, 1983Paper

Preparation of F(ab')2 fragments from mouse IgG of various subclasses

Journal of Immunological Methods
E Lamoyi, A NISONOFF

Abstract

We have investigated the effects of peptic digestion on mouse monoclonal immunoglobulins of each subclass of IgG. F(ab')2 fragments were obtained in good yield from IgG1, IgG2a and IgG3 proteins. The relative rates of digestion were IgG3 greater than IgG2a greater than IgG1. Variations in rate of digestion were noted for individual monoclonal IgG3 proteins. Five different IgG2b proteins were degraded very rapidly without the detectable formation of antigen-binding F(ab')2 fragments. One IgG1 protein out of four tested was also rapidly degraded.

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Citations

Nov 19, 1989·Molecular and Cellular Biochemistry·G T Stevenson
Jan 1, 1990·European Journal of Nuclear Medicine·K Z WalkerG R Boniface
Jan 1, 1993·Cancer Immunology, Immunotherapy : CII·S J CurnowG T Stevenson
Jul 24, 1986·Journal of Immunological Methods·J M Bidlack, P C Mabie
Jun 13, 1988·Journal of Immunological Methods·R KurkelaP Vihko
Jun 2, 1989·Journal of Immunological Methods·S J BoguslawskiR A Fredrickson
Apr 8, 1991·Journal of Immunological Methods·S Haba, A Nisonoff
Jun 18, 1993·Journal of Immunological Methods·C CréminonJ Grassi
Dec 1, 1988·Molecular Immunology·M A Dombrink-Kurtzman, E W Voss
Nov 1, 1993·Molecular Immunology·A BernemanS Avrameas
Jan 1, 1991·International Journal of Immunopharmacology·G J BrunnP R Pentel
Jan 17, 2002·Journal of Immunological Methods·David S WilsonSteffen Nock
Mar 14, 2013·Journal of Agricultural and Food Chemistry·Pan HuShiying Lu
Apr 29, 1999·Scandinavian Journal of Immunology·U Pacheco-GarcíaF J Sánchez-García
May 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·D HolmbergA Coutinho
Jul 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M A SoosK Siddle
Aug 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·B M MuellerS D Gillies
May 7, 2002·Hybridoma and Hybridomics·Leslie A KhawliAlan L Epstein

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