Preparation of fatty acid methyl esters from lipoprotein and macrophage lipid subclasses on thin-layer plates

Lipids
Wolfgang SattlerG M Kostner

Abstract

A simple, accurate, and fast procedure for quantitative analysis of fatty acids (FA) in simple lipid subclasses from different biological specimens is presented. Lipid extracts of isolated plasma lipoproteins (very low, low, and high density lipoproteins; VLDL, LDL, and HDL, respectively) and permanent J774 mouse macrophages were fractionated into lipid subclasses by thin-layer chromatography (TLC) on silica gel 60 plates. Bands comigrating with authentic lipid standards were scraped off under argon and subjected to direct, in situ transesterification with BF3/MeOH in the presence of the TLC adsorbent. Fatty acid methyl esters were subsequently quantitated by capillary gas chromatography. A comparison of the FA content present in total lipid extracts and in lipid subclasses separated by TLC revealed recoveries ranging from 93 (J774 cell extracts) to 99.7% (LDL). The method described is applicable for the measurement of FA in individual lipid subclasses and was successfully applied to quantitatively analyze the FA composition of the phospholipid, triacylglycerol, and cholesteryl ester fraction derived from VLDL, LDL, and HDL. In J774 lipid extracts, the FA composition of the phospholipid-, monoacylglycerol-, diacylglycerol-, fre...Continue Reading

References

Sep 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·S K BasuM S Brown
Nov 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·V W BowryR Stocker
Jan 4, 1991·Biochimica Et Biophysica Acta·W SattlerH Esterbauer
Nov 1, 1990·Lipids·A OhtaW E Lands
Apr 6, 1989·The New England Journal of Medicine·D SteinbergJ L Witztum
Oct 1, 1989·The Journal of Clinical Investigation·S Ylä-HerttualaD Steinberg
Jan 1, 1986·Methods in Enzymology·M J Chapman
Jan 1, 1983·Methods in Enzymology·J L GoldsteinM S Brown
Mar 19, 1981·Clinica Chimica Acta; International Journal of Clinical Chemistry·J M LillingtonH L Makin
Aug 1, 1959·Canadian Journal of Biochemistry and Physiology·E G BLIGH, W J DYER

❮ Previous
Next ❯

Citations

May 22, 2010·Analytical and Bioanalytical Chemistry·Michael A JansenEdith Porter
Feb 13, 2001·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·P W WiesenfeldM W O'Donnell
May 16, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·M VeckaA Zák
Feb 13, 2001·Kidney International. Supplement·J M RoobB M Winklhofer-Roob
Sep 29, 2011·Journal of Neurochemistry·Karoline EtschmaierUte Panzenboeck
Sep 12, 2006·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Caryl J AntalisJohn R Burgess
Mar 26, 2005·BMC Biochemistry·Jing X Kang, Jingdong Wang
Jan 15, 2008·Metabolism: Clinical and Experimental·Anthony LucasFrançoise Goirand
Feb 4, 2006·Journal of Gastrointestinal Surgery : Official Journal of the Society for Surgery of the Alimentary Tract·Matthew I GoldblattHenry A Pitt
Sep 10, 2013·Microbiological Research·Natalia S PaulucciMirta García de Lema
Mar 16, 2007·Plant Physiology and Biochemistry : PPB·Andréina LaffargueStéphane Dussert
Jan 11, 2011·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·Ganapathy Saravanan, Ponnusamy Ponmurugan
Jun 12, 2009·Prostaglandins, Leukotrienes, and Essential Fatty Acids·A H MetherelK D Stark
Jun 14, 2005·Chemico-biological Interactions·Oddrun Anita GudbrandsenRolf K Berge
Jan 9, 2003·The Journal of Laboratory and Clinical Medicine·Alain PiolotSuzanne Lussier-Cacan
Dec 6, 2011·The Journal of Nutritional Biochemistry·Marcelo MardonesGladys Tapia
May 11, 2015·The Science of the Total Environment·Stéphane FirminAnissa Lounès-Hadj Sahraoui

❮ Previous
Next ❯

Related Concepts

Related Feeds

ApoE, Lipids & Cholesterol

Serum cholesterol, triglycerides, apolipoprotein B (APOB)-containing lipoproteins (very low-density lipoprotein (VLDL), immediate-density lipoprotein (IDL), and low-density lipoprotein (LDL), lipoprotein A (LPA)) and the total cholesterol/high-density lipoprotein (HDL) cholesterol ratio are all connected in diseases. Here is the latest research.