PMID: 6971873Mar 1, 1980Paper

Preparation of frog myosin. Isolation and characterization of the light chains

Journal of Muscle Research and Cell Motility
B Focant, F Huriaux

Abstract

Frog myosin can be prepared with a good yield by precipitation of a high ionic strength extract between I 0.20 and 0.05 or by ammonium sulphate fractionation of actomyosin in the presence of Mg-ATP. Two alkali light chains, LC1 and LC3, along with one DTNB light chain LC2 have been isolated by chromatography on ion exchange cellulose after urea dissociation. A supplementary light chain LC1d present in variable amounts from one preparation to the other corresponds to a proteolysis product of LC1. Their stoichiometry, molecular weight, amino acid composition, isoelectric point and peptide map have been determined. Their general proportions and structural properties show many similarities with rabbit skeletal muscle light chains. Amino acid compositions and peptide maps confirm that the additional band LC1d comes from a proteolytic degradation affecting the N-terminal part of LC1.

References

Jul 15, 1976·Journal of Molecular Biology·J Kendrick-JonesA G Szent-Györgyi
Apr 1, 1978·The Biochemical Journal·M A FerencziA G Weeds
Dec 20, 1978·Biochimica Et Biophysica Acta·A Giambalvo, P Dreizen
Oct 21, 1968·Biochimica Et Biophysica Acta·M BárányG Bailin
Nov 14, 1971·Journal of Molecular Biology·A G Weeds, S Lowey
May 15, 1974·European Journal of Biochemistry·G Frank, A G Weeds
Jul 1, 1956·Biochimica Et Biophysica Acta·G HAMOIR, A REUTER
Sep 1, 1961·The Biochemical Journal·J J CONNELL
Aug 1, 1965·Analytical Chemistry·J V BENSON, J A PATTERSON
May 6, 1960·Biochimica Et Biophysica Acta·G HAMOIRM B SMITH

❮ Previous
Next ❯

Citations

Dec 27, 2011·The Journal of Physiology·R ElangovanG Piazzesi
Jun 1, 1987·Journal of Muscle Research and Cell Motility·J Lännergren
Jun 29, 1984·Journal of Chromatography·I Syrový
Jan 1, 1983·Comparative Biochemistry and Physiology. A, Comparative Physiology·I A Johnston

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Archives internationales de physiologie et de biochimie
F Huriaux, B Focant
Journal of Muscle Research and Cell Motility
F HuriauxB Focant
Journal of Muscle Research and Cell Motility
L Dalla LiberaJ H Collins
© 2021 Meta ULC. All rights reserved