PMID: 6537744Jan 1, 1984Paper

Preparation of human spermatozoa for in vitro fertilization by isopycnic centrifugation on self-generating density gradients

Archives of Andrology
V N Bolton, P R Braude

Abstract

A simple and rapid method is described for the retrieval of highly motile, morphologically normal spermatozoa from human semen. This method is a modification of a technique described previously and employs the process of isopycnic centrifugation of semen on self-generating Percoll density gradients. The procedure is carried out under sterile conditions and has no detectable deleterious effects on the fertilizing capacity of spermatozoa. Spermatozoa may be recovered from semen samples of widely differing quality and can be used successfully for in vitro fertilization (IVF). This technique may be useful not only for the preparation of spermatozoa for IVF and possibly for artificial insemination by husband (AIH) but also for the investigation and analysis of the causes of infertility associated with oligozoospermia.

References

Jun 1, 1976·Fertility and Sterility·A LopataB James
Dec 14, 1973·Nature·R J EricssonM Nishino
Sep 1, 1980·British Journal of Obstetrics and Gynaecology·R G EdwardsJ M Purdy
Mar 1, 1982·Journal of Reproduction and Fertility·A O TrounsonJ F Leeton
Oct 1, 1982·Fertility and Sterility·J K Sherman, W P Dmowski

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Citations

Jan 1, 1987·Archives of Andrology·S OshioH Mohri
Jan 1, 1989·Archives of Andrology·M Pardo, N Bancells
Feb 1, 1993·Journal of Assisted Reproduction and Genetics·K Purvis, I Egdetveit
Jan 1, 1987·Archives of Andrology·S OshioH Mohri
May 10, 2008·Expert Review of Proteomics·Florian TriblKatrin Marcus
Jan 20, 2006·Reproduction in Domestic Animals = Zuchthygiene·J M Morrell
Jun 1, 1987·Journal of in Vitro Fertilization and Embryo Transfer : IVF·C A Yates, D M De Kretser
Aug 25, 2017·Zygote : the Biology of Gametes and Early Embryos·J M Morrell, I Mayer
Mar 1, 1994·Baillière's Clinical Obstetrics and Gynaecology·S FlemingS Fishel
May 22, 2008·Reproductive Biomedicine Online·Deshira Saiti, Orly Lacham-Kaplan

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