PMID: 26320483Sep 1, 2015Paper

Preparation of Immunotoxin Herceptin-Botulinum and Killing Effects on Two Breast Cancer Cell Lines

Asian Pacific Journal of Cancer Prevention : APJCP
Saieh HajighasemlouMohammad M Farajollahi

Abstract

Worldwide, breast cancer is the most common cancer diagnosed among women and a leading cause of cancer deaths. The age of onset in Iran has become reduced by a decade for unknown reasons. Herceptin, a humanized monoclonal antibody, is a target therapy for breast cancer cells with over expression of HER2- neu receptors, but it is an expensive drug with only 20% beneficial rate of survival. This study introduces a novel approach to enhance the efficacy of this drug through immunoconjugation of the antibody to botulinum toxin. Decreasing the cost and adverse effects of the antibody were secondary goals of this study. Botulinum toxin was conjugated with Herceptin using heterobifunctional cross linkers, succinimidyl acetylthiopropionate (SATP) and sulfo-succinimidyl-4-(N-maleimidomethyl) cyclohexane-1-carboxylate (SMCC) according to the supplier's guidelines and tested on two breast cancer cell lines: SK-BR-3 and BT-474. Toxin and Herceptin were also used separately as controls. The cytotoxicity assay was also performed using the new bioconjugate on cultured cells with Alamar blue and a fluorescence plate reader. Herceptin-Toxin bioconjugation significantly improved Herceptin efficacy on both breast cancer cell lines when compared t...Continue Reading

References

Sep 29, 2000·Stem Cells·J P Houchins
Nov 28, 2002·The British Journal of Dermatology·M K NaumannUNKNOWN Botox Hyperhidrosis Clinical Study Group
Jun 25, 2003·Expert Review of Anticancer Therapy·Michael UntchKerstin Hermelink
Aug 12, 2003·Advances in Experimental Medicine and Biology·Joan AlbanellPere Gascón
Mar 2, 2005·International Journal of Epidemiology·Michelle D AlthuisLouise A Brinton
Sep 20, 2008·FEMS Immunology and Medical Microbiology·Tohru YoneyamaKoichi Niwa
Jul 8, 2011·Journal of Experimental & Clinical Cancer Research : CR·Xu WeiLiu Zheng
Apr 17, 2012·Asian Pacific Journal of Cancer Prevention : APJCP·Afsoon TaghaviMohamad Amin Pourhoseingholi
Apr 30, 2013·Asian Pacific Journal of Cancer Prevention : APJCP·Cindy BandalaMaricruz Anaya-Ruiz
Oct 12, 2013·CA: a Cancer Journal for Clinicians·Carol DeSantisAhmedin Jemal
Jan 1, 2014·Asian Pacific Journal of Cancer Prevention : APJCP·Aboulfazl AfsharfardEisa Tahmasbpour
Jun 1, 1993·In Vitro Cellular & Developmental Biology. Animal·P Ray

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Citations

Jan 18, 2020·Toxins·Shivam Om Mittal, Bahman Jabbari
May 28, 2019·The Breast Journal·Mohsen MousaviBehrooz Johari

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