Preparation of intact bovine tail intervertebral discs for organ culture.

Journal of Visualized Experiments : JoVE
S C W Chan, B Gantenbein-Ritter

Abstract

The intervertebral disc (IVD) is the joint of the spine connecting vertebra to vertebra. It functions to transmit loading of the spine and give flexibility to the spine. It composes of three compartments: the innermost nucleus pulposus (NP) encompassing by the annulus fibrosus (AF), and two cartilaginous endplates connecting the NP and AF to the vertebral body on both sides. Discogenic pain possibly caused by degenerative intervertebral disc disease (DDD) and disc herniations has been identified as a major problem in our modern society. To study possible mechanisms of IVD degeneration, in vitro organ culture systems with live disc cells are highly appealing. The in vitro culture of intact bovine coccygeal IVDs has advanced to a relevant model system, which allows the study of mechano-biological aspects in a well-controlled physiological and mechanical environment. Bovine tail IVDs can be obtained relatively easy in higher numbers and are very similar to the human lumbar IVDs with respect to cell density, cell population and dimensions. However, previous bovine caudal IVD harvesting techniques retaining cartilaginous endplates and bony endplates failed after 1-2 days of culture since the nutrition pathways were ob...Continue Reading

References

Jun 4, 2008·Spine·Casey L KoreckiJames C Iatridis
Sep 20, 2008·Tissue Engineering. Part C, Methods·Benjamin Gantenbein-RitterKeita Ito
Dec 6, 2008·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Casey L KoreckiJames C Iatridis
Feb 23, 2010·The Spine Journal : Official Journal of the North American Spine Society·Samantha C W ChanKeita Ito
Feb 22, 2011·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Bernice JimLisbet Haglund
Feb 24, 2011·Spine·Samantha C W ChanBenjamin Gantenbein-Ritter
Apr 7, 2011·Methods in Molecular Biology·Benjamin Gantenbein-RitterSibylle Grad

❮ Previous
Next ❯

Citations

Jan 29, 2013·The Spine Journal : Official Journal of the North American Spine Society·Samantha C W ChanBenjamin Gantenbein-Ritter
Jan 11, 2013·Journal of Tissue Engineering and Regenerative Medicine·C MalonzoB Gantenbein-Ritter
Jun 26, 2018·Journal of Functional Biomaterials·Daniela A FrauchigerBenjamin Gantenbein
Feb 3, 2018·Evidence-based Complementary and Alternative Medicine : ECAM·Jungang SunShu Guang Yu
May 24, 2018·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Daniela A FrauchigerBenjamin Gantenbein

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood Clotting Disorders

Thrombophilia includes conditions with increased tendency for excessive blood clotting. Blood clotting occurs when the body has insufficient amounts of specialized proteins that make blood clot and stop bleeding. Here is the latest research on blood clotting disorders.