Preparation of Lignan-Rich Extract from the Aerial Parts of Phyllanthus niruri Using Nonconventional Methods

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Meselhy R MeselhyAli El-Halawany

Abstract

Phyllanthin and related lignans were found to be responsible, at least in part, for most of the activity of Phyllanthus species. This observation encouraged the authors to develop methods for the preparation of an extract rich in phyllanthin and related lignans from the aerial parts of P. niruri L. Direct extraction with solvents produced extracts with variable yields and contents of lignans. Lignans were identified by LC-ESI-MS analysis as phyllanthin (used as marker substance), hypophyllanthin, phylltetralin, nirtetralin, and niranthin. Extraction with boiling water produced 18.10 g% (w/w) extract with a trace amount of lignans (phyllanthin content of 0.33 ± 0.10 mg/g extract), while extraction with MeOH gave 3.6 g% w/w extract with a low phyllanthin content (3.1 mg/g extract), as determined by HPLC. However, Soxhlet extraction with hexane, CH2Cl2, or acetone gave extracts with low yields (0.82, 1.12, and 3.40 g% w/w, respectively) and a higher phyllanthin contents (36.2 ± 2.6, 11.7 ± 1.68, and 11.7 ± 1.10 mg/g extract, respectively). Extraction quality and efficiency were optimized by adopting the following three different approaches: (1) Alkaline digestion of the plant material with 30% potassium hydroxide yielded 3.1 g% w/...Continue Reading

References

Sep 1, 1985·Journal of Ethnopharmacology·K V SyamasundarH Hikino
Mar 3, 2007·The Journal of Pharmacy and Pharmacology·G BagalkotkarJ Stanslas
Apr 29, 2009·Journal of Ethnopharmacology·Vikneswaran Murugaiyah, Kit-Lam Chan
Mar 1, 2007·Indian Journal of Clinical Biochemistry : IJCB·Mary Chatterjee, Parames C Sil
Dec 15, 2012·Experimental Biology and Medicine·Raimundo Fernandes de Araújo JúniorGerlane Coelho Bernardo Guerra
Jan 1, 2013·ISRN Biotechnology·Bernardo Dias RibeiroMaria Alice Zarur Coelho
Oct 22, 2016·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Menaga IlangkovanSyed Nasir Abbas Bukhari

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