Preparation of Linear Cryogel Arrays as a Microfluidic Platform for Immunochromatographic Assays

Analytical Chemistry
Marc ZinggelerJürgen Rühe

Abstract

We describe a new microfluidic platform to perform immunochromatographic assays. The platform consists of a linear assembly of small, porous cryogel monoliths functionalized with various biomolecules. The cryogels are anchored in an optically transparent capillary, which serves as the microfluidic carrier. This assembly enables fluid flow by capillary action and simple optical detection. Using an in situ preparation method, individual compartments are generated from small plugs of polymer solutions that are transformed into small individually functionalized cryogel monoliths through a photoinduced cross-linking reaction. In the same reaction step, the monoliths are firmly anchored to the surface of the capillary. As proof-of-concept, a prototype platform is successfully used for the detection of the inflammatory marker interleukin 6 via a sandwich immunoassay. We observe excellent assay performance metrics that include high sensitivity, good linearity, and low variation. We also demonstrate fluid transport solely by passive means, which is a critical attribute for point-of-care diagnostics.

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Citations

Dec 1, 2017·ACS Applied Materials & Interfaces·Leixiao YuRainer Haag
Apr 7, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Roland Hönes, Jürgen Rühe
Sep 3, 2021·Analytical Chemistry·Patrick L Fosso TeneJürgen Rühe
Sep 11, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Patrick L Fosso TeneJürgen Rühe

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