Preparation of magnetic immobilized metal affinity separation media and its use in the isolation of proteins

Journal of Chromatography. a
T Abudiab, R R Beitle

Abstract

A new method of pseudobiospecific protein isolation is developed and tested, which employs both metal affinity and magnetism as the basis for isolation. The chelating group iminodiacetic acid (IDA) has been coupled to the surface of magnetic agarose, and when charged with metal ions (Cu2+ or Zn2+) is capable of binding model proteins which display metal affinity, and of separating protein mixtures. Magnetic properties of the medium facilitated the batch recovery of the adsorbent, as losses are minimized by concentrating and retaining the separation medium with the aid of a magnet. Model proteins were used to characterize protein adsorption, capacity, and stability of IDA magnetic agarose. Recovery from a cell lysate was demonstrated by protein isolation from extracts of E. coli containing a target protein. Overall, this study effectively illustrates the engineering of separation media which combine several desired properties for the development of a new branch of metal affinity-based bioseparation.

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Citations

Feb 20, 2004·Journal of Colloid and Interface Science·Z G PengM S Uddin
Nov 6, 2001·Journal of Biochemical and Biophysical Methods·V Gaberc-Porekar, V Menart
Sep 6, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Adil DenizliErhan Pişkin
Nov 30, 2004·Biomagnetic Research and Technology·Ivo Safarik, Mirka Safarikova
Jan 20, 2015·Biotechnology and Applied Biochemistry·Işık PerçinAdil Denizli
Jul 21, 2004·Colloids and Surfaces. B, Biointerfaces·Z G PengM S Uddin
Oct 3, 2006·Journal of Colloid and Interface Science·N ShamimM S Uddin
Jun 8, 2006·Journal of Colloid and Interface Science·Misara Hamoudeh, Hatem Fessi
Mar 11, 2006·Biomolecular Engineering·Kalyani Mondal, Munishwar N Gupta
Feb 7, 2004·Biotechnology Progress·Sibel EmirAdil Denizli
Nov 26, 2013·Materials Science & Engineering. C, Materials for Biological Applications·Xueyan ZouChunpeng Song
Dec 6, 2011·Journal of Colloid and Interface Science·Md Shahidul IslamAjoy Kumar Das
Jan 18, 2006·Journal of Biomaterials Science. Polymer Edition·Evrim Banu AltintaşAdil Denizli
Oct 14, 2013·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Xueyan ZouChunpeng Song
Jun 26, 2013·Biomedit︠s︡inskai︠a︡ khimii︠a︡·A S IvanovA I Archakov

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