PMID: 7529416Sep 1, 1994Paper

Preparation of monoclonal antibodies specific for the subclasses of canine IgG

Research in Veterinary Science
G MazzaW P Duffus

Abstract

Canine IgG is composed of four subclasses, which are defined as IgG1, IgG2, IgG3 and IgG4 on the basis of data from fast protein liquid chromatography, and their electrophoretic mobilities and relative concentrations in serum. This paper describes the preparation of mAbs specific for determinants on canine IgG2, IgG3 and IgG4. The mAb specific for IgG2 resulted from a conventional immunisation protocol. The mAb specific for IgG3 was a result of immunisation with IgG3 combined with the suppression of the immune response to IgG1 by passively administered anti-IgG1 antibody. The mAb specific for IgG4 resulted from immunisation with Fab or Fc fragments which were obtained by the cleavage of the IgG4 molecule with papain. The specificity of each mAb was established by using an enzyme-linked immunosorbent assay which showed that all three specific clones recognised a determinant in the Fd region of the canine immunoglobulin molecule.

References

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Citations

Feb 1, 1997·Journal of Comparative Pathology·M J Day
Jun 29, 2002·Veterinary Immunology and Immunopathology·C ArceD Llanes
Jan 8, 1999·Veterinary Immunology and Immunopathology·T M Lian, R E Halliwell
Oct 3, 1999·Veterinary Immunology and Immunopathology·M J Day
Nov 4, 2008·Journal of Veterinary Internal Medicine·L J DavisonB Catchpole
Nov 26, 2013·Veterinary Immunology and Immunopathology·Lisa M BergeronGraeme Bainbridge
Oct 21, 2011·Revista Do Instituto De Medicina Tropical De São Paulo·Flávia Coelho RibeiroMauro C A Marzochi
Sep 1, 1996·Research in Veterinary Science·M J DayS E Shaw
Apr 7, 2009·Veterinary Parasitology·Trícia M F S OliveiraRosangela Z Machado
Oct 31, 2020·The American Journal of Tropical Medicine and Hygiene·Jeffrey W PriestVitaliano Cama
Nov 4, 2020·Scientific Reports·A I Olías-MoleroJ M Alunda

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