PMID: 30236210Sep 22, 2018Paper

Preparation of multivalent egg yolk IgY antibodies against Helicobacter pylori

Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology
Lijin SunZaixin Li

Abstract

Objective To prepare a specific multivalent immunoglobulin of yolk (IgY) against multi-virulence factors of Helicobacter pylori (Hp). Methods The purified recombinant BabA2, UerB and FlaA of Hp were mixed equally with adjuvant, and then used to immunize egg-laying hens to produce specific multivalent IgY. Next, the biological characteristics of purified IgY were analyzed by SDS-PAGE, Western blot and ELISA. To analyze the inhibition effects of multivalent IgY on the growth of Hp in vitro, Hp was co-cultured with different final concentration of IgY (1, 5, 10) mg/mL in the presence or absence of antibiotics. Results After immunization, the titer of egg yolk IgY reached up to 1:150 000. Furthermore, the bacteriostasis assay showed that the multivalent IgY had a significant inhibitory effect on the growth of Hp at 5 mg/mL, and the combination with amoxicillin could further inhibited the growth of Hp. Conclusion The specific multivalent IgY against Hp is successfully prepared.

Related Concepts

Related Feeds

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.