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Preparation of next-generation DNA sequencing libraries from ultra-low amounts of input DNA: Application to single-molecule, real-time (SMRT) sequencing on the Pacific Biosciences RS II.

bioRxiv

Mar 25, 2014

Castle RaleyDwight V. Nissley

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Abstract

We have developed and validated an amplification-free method for generating DNA sequencing libraries from very low amounts of input DNA (500 picograms - 20 nanograms) for single-molecule sequencing on the Pacific Biosciences (PacBio) RS II sequencer. The common challenge of high input r...read more

Mentioned in this Paper

DNA
Drug Carriers
DNA Sequence
Nucleic Acid Amplification Tests
Research Activities
SMRT protein
cDNA Library
Sequence Determinations, DNA
Plasma membrane-bound protein complex
Nucleic Acid Sequencing
51
37
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  • Preparation of next-generation DNA sequencing libraries from ultra-low amounts of input DNA: Application to single-molecule, real-time (SMRT) sequencing on the Pacific Biosciences RS II.

    bioRxiv

    Mar 25, 2014

    Castle RaleyDwight V. Nissley

    PMID: 990003566

    DOI: 10.1101/003566

    Abstract

    We have developed and validated an amplification-free method for generating DNA sequencing libraries from very low amounts of input DNA (500 picograms - 20 nanograms) for single-molecule sequencing on the Pacific Biosciences (PacBio) RS II sequencer. The common challenge of high input r...read more

    Mentioned in this Paper

    DNA
    Drug Carriers
    DNA Sequence
    Nucleic Acid Amplification Tests
    Research Activities
    51
    37

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