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Preparation of next-generation DNA sequencing libraries from ultra-low amounts of input DNA: Application to single-molecule, real-time (SMRT) sequencing on the Pacific Biosciences RS II

bioRxiv

Mar 25, 2014

Castle RaleyDwight Nissley

Abstract

We have developed and validated an amplification-free method for generating DNA sequencing libraries from very low amounts of input DNA (500 picograms – 20 nanograms) for singlemolecule sequencing on the Pacific Biosciences (PacBio) RS II sequencer. The common challenge of high input re...read more

Mentioned in this Paper

Nucleic Acid Amplification Tests
Gene Amplification Technique
Sequencing
NCOR2 wt Allele
Gene Amplification Abnormality
Research Activities
Single Molecule Imaging
SMRT protein
Massively-Parallel Sequencing
Resistant Starch
51
38
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Preparation of next-generation DNA sequencing libraries from ultra-low amounts of input DNA: Application to single-molecule, real-time (SMRT) sequencing on the Pacific Biosciences RS II

bioRxiv

Mar 25, 2014

Castle RaleyDwight Nissley

PMID: 990003566

DOI: 10.1101/003566

Abstract

We have developed and validated an amplification-free method for generating DNA sequencing libraries from very low amounts of input DNA (500 picograms – 20 nanograms) for singlemolecule sequencing on the Pacific Biosciences (PacBio) RS II sequencer. The common challenge of high input re...read more

Mentioned in this Paper

Nucleic Acid Amplification Tests
Gene Amplification Technique
Sequencing
NCOR2 wt Allele
Gene Amplification Abnormality
Research Activities
Single Molecule Imaging
SMRT protein
Massively-Parallel Sequencing
Resistant Starch
51
38

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