Preparation of Nonhuman Primate Eyes for Histological Evaluation After Retinal Gene Transfer

Human Gene Therapy Methods
Peter BellJames M Wilson

Abstract

To evaluate gene therapy for retinal disorders, appropriate models of the human eye are needed. Nonhuman primate eyes offer significant advantages over rodent eyes. However, current preparation methods have limitations. Here, a protocol is described for histological processing of nonhuman primate eyes after gene transfer. The user dissects unfixed eyes, flattens the globe parts within filter paper, and performs formalin fixation and paraffin embedding. This method obviates the need for harsh fixatives, allowing subsequent immunostaining or in situ hybridization while preserving tissue integrity for histopathological evaluation. Moreover, the straight orientation of the retinal cell layers is ideal for image analysis.

References

Sep 1, 1991·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·L M Weiss, Y Y Chen
Jan 1, 1965·Stain Technology·E SUTTER, W MEIER-RUGE
May 20, 2004·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Touji KimuraJunzo Sasaki
Jun 24, 2011·Science Translational Medicine·Luk H VandenbergheJean Bennett
Feb 6, 2013·PloS One·Luk H VandenbergheJames M Wilson
Oct 19, 2016·Human Gene Therapy·Pavitra S RamachandranJean Bennett
Jan 13, 2018·Human Gene Therapy·Livia S CarvalhoLuk H Vandenberghe

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Methods Mentioned

BETA
dissection
biopsy

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