Preparation of nonradioactive probes for in situ hybridization

Methods : a Companion to Methods in Enzymology
T W Abraham

Abstract

In situ hybridization (ISH) enables the precise localization of RNA targets and provides an avenue to study the temporal and spatial patterns of expression of specific genes. ISH has evolved from being an esoteric technique to one that is routinely used by researchers in many areas of research. A major driving force has been the development of numerous nonisotopic labeling and signal detection methods. Historically, radioactive probes and autoradiography provided sensitivity that was unattainable with nonisotopic probes. But the long exposure times required for signal detection and the perceived dangers associated with radioactivity limit its use. Advances in nonisotopic detection systems have overcome many of the limitations associated with using radiolabeled probes. One of the most significant contributions from nonisotopic methods is the ability to discriminate between multiple nucleic acid sequences simultaneously.

References

Jan 1, 1992·European Journal of Pediatrics·S E Jaeggi-GroismanE R Froesch
Sep 11, 1985·Nucleic Acids Research·E T Schenborn, R C Mierendorf
Jun 25, 1981·Nucleic Acids Research·L M Angerer, R C Angerer
Apr 25, 1984·Nucleic Acids Research·M Renz, C Kurz
Apr 24, 2001·Methods : a Companion to Methods in Enzymology·J de Almeida EnglerG Engler
Apr 24, 2001·Methods : a Companion to Methods in Enzymology·T Jowett
Apr 24, 2001·Methods : a Companion to Methods in Enzymology·G Hauptmann

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Citations

Apr 24, 2001·Methods : a Companion to Methods in Enzymology·K ButlerJ B Gurdon
Apr 24, 2001·Methods : a Companion to Methods in Enzymology·J de Almeida EnglerG Engler
Apr 24, 2001·Methods : a Companion to Methods in Enzymology·K M Correia, R A Conlon
Apr 24, 2001·Methods : a Companion to Methods in Enzymology·G Hauptmann
Sep 28, 2013·PloS One·Chung Chew HwangTakuya Mizuno
Feb 19, 2002·Advances in Physiology Education·Andrea D WestonGerald M Kidder

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