Preparation of oligomeric beta-amyloid 1-42 and induction of synaptic plasticity impairment on hippocampal slices.

Journal of Visualized Experiments : JoVE
Mauro FàOttavio Arancio

Abstract

Impairment of synaptic connections is likely to underlie the subtle amnesic changes occurring at the early stages of Alzheimer s Disease (AD). beta-amyloid (A beta), a peptide produced in high amounts in AD, is known to reduce Long-Term Potentiation (LTP), a cellular correlate of learning and memory. Indeed, LTP impairment caused by A beta is a useful experimental paradigm for studying synaptic dysfunctions in AD models and for screening drugs capable of mitigating or reverting such synaptic impairments. Studies have shown that A beta produces the LTP disruption preferentially via its oligomeric form. Here we provide a detailed protocol for impairing LTP by perfusion of oligomerized synthetic A beta1-42 peptide onto acute hippocampal slices. In this video, we outline a step-by-step procedure for the preparation of oligomeric A beta(1-42;). Then, we follow an individual experiment in which LTP is reduced in hippocampal slices exposed to oligomerized A beta(1-42;) compared to slices in a control experiment where no A beta(1-42;) exposure had occurred.

References

May 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·M P LambertW L Klein
Nov 11, 1999·European Journal of Pharmacology·A ItohT Nabeshima
Sep 24, 2002·Proceedings of the National Academy of Sciences of the United States of America·Ottavio V VitoloMichael Shelanski
Dec 25, 2002·The Journal of Biological Chemistry·W Blaine StineMary Jo LaDu
Jul 4, 2008·The Journal of Clinical Investigation·Fabrizio TrincheseOttavio Arancio
Jan 2, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Daniela PuzzoOttavio Arancio

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Citations

Dec 2, 2011·Biochemistry·Harry LeVineCorinne E Augelli-Szafran
Sep 18, 2012·PloS One·Rekha BhatClaudio Torres
Oct 21, 2015·Journal of Alzheimer's Disease : JAD·Mauro FàOttavio Arancio
Sep 15, 2011·Neurobiology of Learning and Memory·Sergio T Ferreira, William L Klein
May 21, 2015·Brain Structure & Function·Ria UhlemannGolo Kronenberg
Jun 4, 2015·PloS One·Jenny DworzakPhilip M Murphy
Oct 31, 2019·Scientific Reports·Hae Nim LeeJihye Seong
Nov 30, 2019·Oxidative Medicine and Cellular Longevity·Yan LiuYunlong Zhang

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