Preparation of porous bioactive ceramic microspheres and in vitro osteoblastic culturing for tissue engineering application

Acta Biomaterialia
S-J HongH-W Kim

Abstract

Microparticulates are useful for directly filling defective tissues as well as for delivering cells and bioactive molecules in regenerative medicine. This paper reports on the production of bioactive ceramic microspheres with an interconnected macropore structure. The sol-gel derived calcium silicate powder was homogenized with an oligomeric Camphene melt, which was used as a novel porogen, and spherical-shaped microparticulates were obtained by an oil-in-water emulsion method. A porous structure was generated through the sublimation of Camphene within the calcium silicate-Camphene solidified blend under ambient conditions. The microspheres retained the crystalline phase of apatite and wollastonite during heat treatment and induced calcium phosphate precipitation under a body-simulating medium, showing the characteristics of bone-bioactive materials. Osteoblastic cells were observed to anchor to and spread well over the surface of the porous microspheres, and further to proliferate actively with culturing time. The bioactive and porous microspheres developed are considered potentially useful in the regeneration of hard tissues as a matrix for tissue engineering as well as a direct filling material.

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Citations

Nov 7, 2012·Tissue Engineering. Part B, Reviews·Jeong-Hui ParkIvan B Wall
Jun 27, 2013·PloS One·Firouzeh SabriNicholas Leventis
Sep 30, 2010·Journal of Biomedical Materials Research. Part a·Gráinne M CunniffeGlenn R Dickson
Jun 30, 2011·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Jung-Ho YangTae-Yub Kwon
May 18, 2011·Biotechnology Progress·Mariana B Oliveira, João F Mano
May 9, 2012·Journal of Biomedical Materials Research. Part a·Hye-Sun YuHae-Won Kim
Apr 1, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Chukwuma E Nweke, Jan P Stegemann

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