PMID: 1739Nov 1, 1975

Preparation of proteolytic enzymes from the thermophilous actinomycete Actinomyces thermovulgaris str. T-54

Prikladnaia biokhimiia i mikrobiologiia
S N Vybornykh, N S Egorov


A preparation with a high proteolytic activity has been obtained by acetone precipitation from the culture liquid filtrate of the thermophilous actinomycete Actinomyces thermovulgaris str. T-54. The proteolytic (caseinolytic), fibrinolytic and thrombolytic activity of the preparation is comparable with that of trypsin and far superior to that of fibrinolysine. The preparation is stable at pH 5.0=9.5 and inactivated in the acid zone. The study of pH dependent proteolytic activity has shown acid, neutral and alkaline proteases in the preparation. It is relatively thermostable, and is completely inactivated for 10 min at 90 degrees. It is suggested that the preparation contains four enzymes or four enzymic groups that are different in their temperature sensitivity.

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Actinomycosis is a rare, chronic, and slowly progressive granulomatous disease caused by filamentous Gram positive anaerobic bacteria from the Actinomycetaceae family (genus Actinomyces). The disease is characterised by the formation of painful abscesses in the mouth, lungs,breast or gastrointestinal tract. Discover the latest research on actinomycosis here.