Abstract
In order to facilitate the use of proteosome-based vaccines, we have identified and analyzed the parameters that affect their immunogenicity. As a model system we used synthetic peptides (LCF6) containing sequences from the immunodominant (NANP)n tandem repeat region of the P. falciparum circumsporozoite protein, hydrophobically complexed to multimeric protein preparations (proteosomes) of meningococcal outer membrane proteins (OMP), since we have previously shown that high levels of anti-(NANP)n IgG can be elicited in mice by use of this novel adjuvant system (Lowell et al., 1988a). We have now examined these preparations by velocity sedimentation and measured their ability to elicit an IgG response in mice. Velocity sedimentation of freshly mixed OMP and LCF6, without dialysis, produced a limited number of small complexes, whereas dialysis of the mixture for 4 d yielded heterogeneously sized complexes that became more homogeneous when the dialysis was carried out for 7 or 10 days. The most homogeneous of these peptide-proteosome complexes (those dialyzed for 10 days) induced substantial levels of anti-(NANP)n IgG in mice, and shorter periods of dialysis resulted in vaccines that induced proportionately lower titers. Analysis ...Continue Reading
References
May 1, 1979·The Journal of Clinical Investigation·W D ZollingerS Berman
Dec 14, 1978·Nature·B MoreinV Schirrmacher
Jul 16, 1987·Nature·D A HerringtonD Gillessen
May 6, 1988·Science·G H LowellW T Hockmeyer
Jun 6, 1987·Lancet·W R BallouJ F Young
May 24, 1985·Science·J F YoungC L Diggs
Jan 1, 1988·Annual Review of Public Health·G I Higashi
Feb 1, 1988·The Journal of Experimental Medicine·G H LowellW D Zollinger
Mar 17, 1988·Nature·B Morein
Aug 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J P Tam
Jul 1, 1983·The Journal of General Virology·B MoreinK Simons
Jan 1, 1983·Annual Review of Microbiology·T M ShinnickR A Lerner
Jan 1, 1984·Molecular Immunology·T P Hopp
Oct 1, 1984·Infection and Immunity·W D ZollingerB Brandt
Mar 4, 1984·Nature·B MoreinA Osterhaus
Jun 1, 1980·The Journal of Experimental Medicine·P PotocnjakV Nussenzweig
Dec 1, 1980·Analytical Biochemistry·A J Furth
Citations
May 4, 2005·Journal of Controlled Release : Official Journal of the Controlled Release Society·Isabella SchöllErika Jensen-Jarolim
Sep 29, 1999·Vaccine·F EstevezL E Fernandez
Dec 6, 2011·Vaccine·Wendell D ZollingerCarl R Alving
Nov 16, 1999·Biochimica Et Biophysica Acta·M M ParmarT D Madden
Apr 9, 2017·Scientific Reports·Michael L ReiserLee M Wetzler