Preparation of pseudotyped lentiviral vectors resistant to inactivation by serum complement

Cold Spring Harbor Protocols
Ghiabe H Guibinga, Theodore Friedmann

Abstract

A major obstacle to in vivo delivery of lentivirus or other retroviral vectors is their lability in the presence of serum. In vivo, these viral particles are rapidly destroyed by nonspecific complement-mediated degradation mechanisms. The eventual effective use of retroviral vectors for in vivo gene delivery would be greatly facilitated by the development of methods to protect the viral particles from such degradation. This protocol describes methods for the production of complement-stabilized lentiviral vectors either by pseudotyping the viral particles with a fusion envelope protein containing the complement-regulatory protein CD55 (decay accelerating factor, DAF) or by coassembly with the native DAF protein. An in vitro serum inactivation assay is also described.

References

Oct 15, 1976·Virology·R M WelshM B Oldstone
Oct 16, 1975·Nature·R M WelshM B Oldstone
Feb 26, 1998·Immunopharmacology·M Kirschfink
Sep 14, 2000·Molecular Therapy : the Journal of the American Society of Gene Therapy·N J DePoloT W Dubensky
Feb 8, 2003·Human Gene Therapy·Mukesh KumarJoshua Zimmerberg
Mar 18, 2005·Molecular Therapy : the Journal of the American Society of Gene Therapy·Ghiabe H Guibinga, Theodore Friedmann

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Citations

Oct 24, 2012·BMC Biotechnology·Isabelle HoubrackenLuc Bouwens
Sep 29, 2020·Current Protocols in Molecular Biology·Katherine P Gill, Mark Denham
Nov 22, 2017·Human Gene Therapy Methods·Guillermo GarauletAntonio Rodríguez
Aug 4, 2020·Human Gene Therapy·Yogendra S RajawatHans-Peter Kiem

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