PMID: 86586Jan 1, 1979Paper

Preparation of specific antisera against adenoviruses by affinity bead immunization (ABI)

Journal of Immunological Methods
M E JohanssonL Svensson


Certain adenovirus types can be replicated only to low titer in tissue cultures. Other, such as adenovirus strains associated with infantile gastroenteritis, cannot be replicated in vitro. A method which allows preparation of specific antisera has therefore been evaluated. The procedure involves coupling of group-specific antibodies against adenovirus capsid subunits to CNBr-activated Sepharose 4B; reaction of crude virus suspensions with immobilized adenovirus-specific IgG; elimination of contaminating material by extensive washing using a wide pH range; and immunization with adenovirus immunogens immobilized on the beads. Efficient immunization was obtained with immunogen doses of both 50 ng and 50 microgram. The immunization procedure which has been designated affinity bead immunization (ABI) could therefore have a wide applicability in cases where the relevant immunogen constitutes a minor fraction of a crude preparation.


Jan 1, 1978·Journal of Immunological Methods·M E Johansson, G Wadell
Jan 1, 1976·Journal of Immunological Methods·M E JohanssonM Grandien
May 1, 1977·Journal of Virology·G Winberg, G Wadell
Jan 1, 1976·Journal of Immunological Methods·W HeinzelH Grimminger
Jul 1, 1969·Virology·D O WhiteJ V Maizel
Oct 1, 1969·Japanese Journal of Medical Science & Biology·Y Moritsugu
Sep 1, 1969·The Journal of General Virology·E Norrby
Dec 1, 1970·Journal of Virology·J C Hierholzer, W R Dowdle
Dec 28, 1971·Biochimica Et Biophysica Acta·J I Thorell, B G Johansson

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