PMID: 86586Jan 1, 1979Paper

Preparation of specific antisera against adenoviruses by affinity bead immunization (ABI)

Journal of Immunological Methods
M E JohanssonL Svensson

Abstract

Certain adenovirus types can be replicated only to low titer in tissue cultures. Other, such as adenovirus strains associated with infantile gastroenteritis, cannot be replicated in vitro. A method which allows preparation of specific antisera has therefore been evaluated. The procedure involves coupling of group-specific antibodies against adenovirus capsid subunits to CNBr-activated Sepharose 4B; reaction of crude virus suspensions with immobilized adenovirus-specific IgG; elimination of contaminating material by extensive washing using a wide pH range; and immunization with adenovirus immunogens immobilized on the beads. Efficient immunization was obtained with immunogen doses of both 50 ng and 50 microgram. The immunization procedure which has been designated affinity bead immunization (ABI) could therefore have a wide applicability in cases where the relevant immunogen constitutes a minor fraction of a crude preparation.

References

Jan 1, 1978·Journal of Immunological Methods·M E Johansson, G Wadell
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Citations

Jan 1, 1979·Journal of Medical Virology·P A JacobssonG Wadell
Jan 1, 1986·Archives of Virology·M J Albert
Jan 1, 1983·Journal of Immunological Methods·J C Unkeless, G A Healey
Feb 1, 1984·The Journal of Clinical Investigation·H B FleitJ C Unkeless
Jul 1, 1982·The Journal of Pediatrics·R H YolkenS E Strauss
Feb 1, 1985·Journal of Medical Virology·J P LeiteH G Schatzmayr
Sep 1, 1985·Journal of Medical Virology·M E JohanssonG Wadell
Jan 1, 1980·Annals of the New York Academy of Sciences·G WadellG Sundell
Jan 1, 1983·Journal of Medical Virology·J C de JongR G Firtzlaff
Jun 1, 1982·The Journal of Hygiene·A H KiddC R Madeley
Jan 1, 1983·Microbiology and Immunology·M ShinagawaG Sato
Oct 1, 1979·Journal of Clinical Microbiology·M RetterM Petric
Sep 1, 1984·Journal of Clinical Microbiology·M BrownP J Middleton
Sep 1, 1984·Journal of Clinical Microbiology·I UhnooM E Johansson
Jul 1, 1982·Journal of Clinical Microbiology·J C HierholzerH C Spencer
Jun 1, 1980·Journal of Clinical Microbiology·P HalonenE Torfason
Jan 1, 1990·Journal of Applied Biomaterials : an Official Journal of the Society for Biomaterials·N KossovskyC V Deshpandey

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