PMID: 32677412Nov 1, 2018Paper

Preparation of VEGFR2 Targeted Ultrasound Microbubbles by Freeze-drying and Targeting Study in vitro

Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition
Huan ZhangFeng Yan

Abstract

To prepare vascular endothelial growth factor receptor2 (VEGFR2) targeted ultrasound contrast agent (microbubbles, MBs) by freeze-dried method and to evaluate its contrast enhanced effect and targeting capability through in vitro experiments. Targeted MBs were prepared using the biotin-avidin linkage to conjugate rat anti-mouse VEGFR2 monoclonal antibody to the surface of biotinylated MBs. Morphology, size and distribution of MBs were assessed. The binding of streptavidin (FITC marker) and VEGFR2 monoclonal antibody (PE labeled rabbit IgG) to MBs was verified by immunofluorescence staining. In vitro targeting experiments were performed with human umbilical vein endothelial cells (HUVECs). The binding capacity of MBs to HUVECs were detected by three groups including untargeted MBs group (adding 1×107 untargeted MBs), antibody presaturation added VEGFR2 targeted MBs group (after being incubated with excess VEGFR2 antibody, 1×107 VEGFR2 targeted ultrasound MBs were added) and VEGFR2 targeted MBs group (adding 1×107 VEGFR2 targeted ultrasound MBs). Contrast enhanced effects of VEGFR2 targeted MBs were preliminarily examined using an ultrasound imaging system and a home-made extracorporeal circulating device. The monoclonal antibody...Continue Reading

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