PMID: 6164588May 1, 1981Paper

Preparation of viable islet cells from dogs by a new method

Diabetes
A Horaguchi, R C Merrell

Abstract

A new method for the preparation of viable islet cells from the normal dog pancreas is described, based on the perfusion of the pancreatic duct with collagenase. Exposure of the acinar tissue to the highest concentrations of collagenase results in improved islet yield with decreased acinar contamination. After the selective digestion of the gland by ductal perfusion, mechanical dissociation liberates single cells and clumps. Analysis of the procedure by insulin yield and amylase attrition indicates a 57% B-cell recovery and a sixfold enrichment in B-cell concentration. The cells have been transplanted as autografts into dogs after pancreatectomy. In 5 of 7 transplanted dogs, normoglycemia was achieved postoperatively.

Citations

Apr 1, 1984·World Journal of Surgery·D W Scharp
Sep 1, 1989·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·R Takaki
Jul 8, 2009·Journal of Gastrointestinal Surgery : Official Journal of the Society for Surgery of the Alimentary Tract·Ryan M Thomas, Syed A Ahmad
Oct 1, 1983·The Journal of Surgical Research·A HoraguchiR C Merrell
Mar 1, 1985·The Journal of Surgical Research·L F CobbR C Merrell
Feb 1, 1989·The Journal of Surgical Research·J A van der VlietD E Sutherland
Jan 1, 1992·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·S J Sullivan
Jun 1, 2005·Archives of Medical Research·Jason L GagliaGordon C Weir
Aug 4, 2001·Clinica Chimica Acta; International Journal of Clinical Chemistry·A PileggiL Inverardi
Jul 5, 2003·The Journal of Surgical Research·Takashi KenmochiTakenori Ochiai
Nov 1, 1985·Artificial Organs·R C Merrell, G Basadonna
Nov 1, 1986·The Journal of Clinical Investigation·R AlejandroD H Mintz
Jun 17, 2014·Seminars in Pediatric Surgery·Michael McCall, A M James Shapiro
Oct 1, 1995·Annals of Surgery·D C WahoffD E Sutherland
Jan 1, 1990·Seminars in Surgical Oncology·P J Jung, R C Merrell
May 9, 2006·Treatments in Endocrinology·Wayne Truong, A M James Shapiro
Apr 1, 1984·World Journal of Surgery·R V RajotteN N Kneteman
Apr 1, 1984·World Journal of Surgery·R Downing
Jun 1, 1986·World Journal of Surgery·D W Gray, P J Morris
Aug 1, 1984·World Journal of Surgery·D AldersonI D Johnston
Oct 1, 1986·World Journal of Surgery·R MerrellG Basadonna
Jan 1, 1990·Biomaterials, Artificial Cells, and Artificial Organs·G Reach
Jun 10, 2014·Veterinary Surgery : VS·Daniela VrabelovaAmer Rajab
Dec 1, 1988·The Journal of Surgical Research·M P Corlett, D W Scharp
Oct 1, 1985·The Journal of Surgical Research·N M KnetemanA W Procyshyn
Dec 1, 1990·The Journal of Surgical Research·J DanisU J Hesse
Mar 1, 1988·The Journal of Surgical Research·L RosenbergA I Vinik
Oct 1, 1986·The British Journal of Surgery·D W GrayP J Morris
Sep 1, 1988·American Journal of Surgery·G BasadonnaR C Merrell
Sep 1, 1986·The British Journal of Surgery·S M GriffinJ R Farndon
Jun 1, 1993·Gastroenterology Clinics of North America
Mar 1, 1988·American Journal of Surgery·K KakizakiR Merrell
Oct 24, 1998·General and Comparative Endocrinology·L RuffierN Rideau
Feb 6, 2007·Transplantation Proceedings·P AndradesJ M Thomas
Nov 12, 2016·Nature Reviews. Endocrinology·A M James ShapiroCamillo Ricordi
Nov 1, 1992·The Journal of Surgical Research·P T van SuylichemR van Schilfgaarde
Apr 1, 1983·Cryobiology·D W Scharp, J A Long
Jan 16, 2007·Transplantation·Tatsuya KinJonathan R T Lakey

❮ Previous
Next ❯

Related Concepts

Related Feeds

American Diabetes Association Journals

Discover the latest diabetes research published by the journals from the American Diabetes Association.