Preparation of Vitamin E-Containing High-Density Lipoprotein and Its Protective Efficacy on Macrophages

Assay and Drug Development Technologies
Manman SuTianmin Xu

Abstract

Atherosclerosis is a major cause for cardiovascular diseases. High-density lipoprotein (HDL) may reduce atherosclerosis through several different mechanisms. HDL is composed of lipids, cholesterol, cholesteryl esters, triglycerides, and phospholipids, mainly phosphatidylcholine plus specialized proteins called apolipoproteins (apos). In this study, we prepared vitamin E containing HDL (VE-HDL) that contains egg phosphatidylcholine, cholesterol, vitamin E, and two kinds of recombinant human apolipoproteins (rhapo)-rhapoA-I and rhapoE in vitro by the facilitation of cholate. After that, we studied the effects of VE-HDL on foam cell formation, cellular cholesterol efflux, oxidative low-density lipoprotein (oxLDL)-stimulated oxidative stress, and apoptosis of macrophages to evaluate the protective efficacy of VE-HDL on macrophages. As the results showed, we prepared a new type of reconstituent HDL with apolipoproteins and vitamin E for the first time. VE-HDL has protective efficacy on macrophages. It has the prospect of becoming a therapeutic agent on atherosclerosis in the future.

References

Mar 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·B W ShenF J Kézdy
Sep 1, 1994·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·F LupuE K Kruithof
Jun 17, 2004·Circulation·Gerd Assmann, Antonio M Gotto
Mar 2, 2005·Nature Reviews. Drug Discovery·Patrick Linsel-Nitschke, Alan R Tall
May 14, 2005·Current Opinion in Lipidology·Olga Gursky
Jun 24, 2009·Protein Expression and Purification·Manman SuWeiqun Yan
Oct 3, 2009·Arteriosclerosis, Thrombosis, and Vascular Biology·Laurent Yvan-CharvetAlan R Tall
Jul 27, 2012·Journal of Lipid Research·Jason M MeyerDeneys R van der Westhuyzen
Aug 18, 2012·Assay and Drug Development Technologies·Manman SuWeiqun Yan
May 23, 2014·Current Biology : CB·Michael Schieber, Navdeep S Chandel
Dec 9, 2014·Biochimica Et Biophysica Acta·Stjepana KovacAndrey Y Abramov
Jan 16, 2016·Biochemical and Biophysical Research Communications·Qin ShaoBen He

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Citations

Feb 20, 2019·IUBMB Life·Erdi SozenNesrin Kartal Ozer

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Methods Mentioned

BETA
density gradient centrifugation
reverse phase chromatography
MDA

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