Preparation of Yeast Hydrolysate Enriched in Cyclo-His-Pro (CHP) by Enzymatic Hydrolysis and Evaluation of Its Functionality

Preventive Nutrition and Food Science
Hyun Jung LeeHyung Joo Suh

Abstract

In this study, we attempted to enrich cyclo-His-Pro (CHP) using enzymatic hydrolysis of yeast and to evaluate the functionality of yeast hydrolysate (YH)-enriched CHP. Flavourzyme offered a better performance in enhancing CHP content than other proteases. The CHP enrichment conditions were optimized as follows: addition of 1% Flavourzyme, 48-h incubation at 60°C, and pH 6.0. The CHP content significantly increased by 20-fold after ultra-filtration (UF). Maximal CHP translation was obtained after heating for 8 h at 50°C and pH 7.0. YH showed poor foaming capacity between pH 3.0 to 9.0. The emulsifying activities of YHs were slightly higher at near acidic pH. Increase in heating temperature and time resulted in decreased CHP content. The results indicate that YH is more heat stable after UF. Therefore, the CHP in YH after UF can be used as a food additive with physiological CHP activity and high heat stability.

References

Oct 1, 1989·Neuropeptides·H SteinerR T Rosenkranz
Jan 1, 1981·Critical Reviews in Food Science and Nutrition·Z E Sikorski, M Naczk
Apr 6, 1981·Brain Research·J E MorleyC Prasad
Apr 1, 1996·Journal of the American College of Nutrition·H MizumaC W Hilton
Dec 10, 1999·Journal of Chromatography. a·R KubecJ Velísek
Feb 15, 2001·Metabolism: Clinical and Experimental·M K SongV L Go
Jun 26, 2002·Letters in Applied Microbiology·J H KohH J Suh
Aug 28, 2003·Diabetes, Obesity & Metabolism·I K HwangM K Song
May 12, 2004·The Journal of Nutritional Biochemistry·Kunio SuetsunaJiun-Rong Chen
Feb 3, 2005·Journal of Agricultural and Food Chemistry·Eresha MendisSe-Kwon Kim
Aug 3, 2005·Bioresource Technology·Tricia L TemplinKent D Rausch
Oct 29, 2005·Metabolism: Clinical and Experimental·Moon K SongDean T Yamaguchi
Nov 30, 2005·Bioresource Technology·Zhenyu LiAran H-Kittikun
May 9, 2009·British Journal of Pharmacology·M K SongE M Cornford
Jan 1, 1994·Bioscience, Biotechnology, and Biochemistry·Y UedaR Miyajima

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Citations

Apr 25, 2020·World Journal of Microbiology & Biotechnology·Zeinab TakallooReza H Sajedi

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