Preparation, optimization, and structures of cross-linked enzyme aggregates (CLEAs)

Biotechnology and Bioengineering
R SchoevaartR A Sheldon

Abstract

The broad applicability of the cross-linking of enzyme aggregates to the effective immobilisation of enzymes is demonstrated and the influence of many parameters on the properties of the resulting CLEAs is determined. The relative simplicity of the operation ideally lends itself to high-throughput methodologies. The aggregation method was improved up to 100% activity yield for any enzyme. For the first time, the physical structures of CLEAs are elucidated.

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Citations

Jun 26, 2009·Applied Microbiology and Biotechnology·Anna MalandraLudmila Martínková
Sep 3, 2011·Applied Microbiology and Biotechnology·Roger A Sheldon
Jan 10, 2008·Bioprocess and Biosystems Engineering·Tina KubitzkiStephan Lütz
Nov 28, 2009·Biotechnology Letters·Ulrich RoesslBernd Nidetzky
May 24, 2012·Biotechnology Letters·Lei ChenMin-Hua Zong
Apr 24, 2013·Applied Biochemistry and Biotechnology·Abhishek BhattacharyaAnjana Sharma
Mar 25, 2014·Colloids and Surfaces. B, Biointerfaces·Yanina N MartínezGuillermo R Castro
Sep 21, 2007·Chemistry Central Journal·Sohel DalalMunishwar Nath Gupta
Aug 29, 2014·Applied Biochemistry and Biotechnology·Jianfeng ZhengJun Zhang
Jan 9, 2014·Applied Biochemistry and Biotechnology·Yanjun JiangJing Gao
Jun 10, 2014·Enzyme and Microbial Technology·Abhishek Bhattacharya, Brett I Pletschke
Jan 25, 2012·Colloids and Surfaces. B, Biointerfaces·Joey N Talbert, Julie M Goddard
Nov 25, 2011·Enzyme and Microbial Technology·Eun-Ah ChoJae-Gu Pan
Mar 8, 2011·Bioresource Technology·María Inmaculada García-GarcíaAlvaro Sánchez-Ferrer
May 12, 2009·Biotechnology Advances·Ludmila MartínkováVladimír Křen
Aug 27, 2015·International Journal of Biological Macromolecules·Joyeeta Mukherjee, Munishwar Nath Gupta
Sep 2, 2008·Trends in Biotechnology·Lorena Betancor, Heather R Luckarift
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Sep 25, 2010·Bioresource Technology·Mengfan WangZhimin He
Sep 3, 2011·Journal of Biotechnology·Stanislav PepeliaevJana Dostálová
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May 31, 2016·Analytical Biochemistry·Joyeeta MukherjeeMunishwar Nath Gupta
Oct 27, 2016·Analytical Chemistry·Mayte Conejero-MurielJosé A Gavira

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